Open Access

ARTICLE

Genome-Wide Analysis of the KANADI Gene Family and Its Expression Patterns under Different Nitrogen Concentrations Treatments in Populus trichocarpa

Minghui Niu^1,#, Heng Zhang^1,#, Xiangyang Li¹, Zhibao Hu¹, Hongjiao Zhang^2,3, Zhiru Xu^2,3, Chunpu Qu^1,4,*, Guanjun Liu^1,*

1 State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University), School of Forestry, Northeast Forestry University, Harbin, 150040, China
2 Key Laboratory of Saline-Alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education, Harbin, 150040, China
3 College of Life Science, Northeast Forestry University, Harbin, 150040, China
4 College of Forestry, Guizhou University, Guiyang, 550025, China

* Corresponding Authors: Chunpu Qu. Email: ; Guanjun Liu. Email:
# The first two authors have contributed equally to this work

Phyton-International Journal of Experimental Botany 2023, 92(7), 2001-2015. https://doi.org/10.32604/phyton.2023.029350

Received 14 February 2023; Accepted 22 March 2023; Issue published 29 May 2023

Abstract

KANADI (KAN) is a plant-specific gene that controlled the polarity development of lateral organs. It mainly acted on the abaxial characteristics of plants to make the lateral organs asymmetrical. However, it had been less identified in woody plants. In this study, the members of the KAN gene family in Populus trichocarpa were identified and analyzed using the bioinformatics method. The results showed that a total of 8 KAN family members were screened out, and each member contained the unique GARP domain and conserved region of the family proteins. Phylogenetic analysis and their gene structures revealed that all KAN genes from P. trichocarpa, Arabidopsis thaliana, and Nicotiana benthamiana could be divided into four subgroups, while the eight genes in P. trichocarpa were classified into three subgroups, respectively. The analysis of tissue-specific expression indicated that PtKAN1 was highly expressed in young leaves, PtKAN6 was highly expressed in young leaves and mature leaves, PtKAN2, PtKAN5, and PtKAN7 were highly expressed in nodes and internodes, PtKAN8 was highly expressed in roots, and PtKAN3 and PtKAN4 showed low expression levels in all tissues. Among them, PtKAN2 and PtKAN6, and PtKAN4 and PtKAN5 might have functional redundancy. Under high nitrogen concentrations, PtKAN2 and PtKAN8 were highly expressed in mature stems and leaves, respectively, while PtKAN4, PtKAN5, and PtKAN7 were highly expressed in roots. This study laid a theoretical foundation for further study of the KAN gene-mediated nitrogen effect on root development.

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Keywords

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