Open Access

ARTICLE

Identification of a Novel OsCYP2 Allele that Was Involved in Rice Response to Low Temperature Stress

by Hongxiu Gao¹, Lin Zhu², Tianqi Liu¹, Xueyu Leng¹, Zhenxing Zhu³, Wei Xie¹, Haitao Lv¹, Zhengxun Jin¹, Ping Wu^4,#, Zhongchen Zhang^1,*

1 College of Agriculture, Northeast Agricultural University, Harbin, 150030, China
2 Hulin Agricultural Technology Extension Center, Hulin, 158400, China
3 Liaoning Academy of Agricultural Sciences, Shenyang, 110161, China
4 Zhejiang University, Hangzhou, 310000, China

* Corresponding Author: Zhongchen Zhang. Email:
# The author died prior to the submission of this paper. Other authors express their great gratitude and remembrance to him

(This article belongs to the Special Issue: Identification of Genetic/Epigenetic Components Responding to Biotic and Abiotic Stresses in Crops)

Phyton-International Journal of Experimental Botany 2023, 92(6), 1743-1763. https://doi.org/10.32604/phyton.2023.026516

Received 09 September 2022; Accepted 12 December 2022; Issue published 11 April 2023

Abstract

Cyclophilin (CYP) plays an important role in plant response to stress, and OsCYP2, one gene of cyclophlilin family, is involved in auxin signal transduction and stress signaling in rice. However, the mechanism that OsCYP2 is involved in rice response to low temperature is still unclear. We identified a new OsCYP2 allelic mutant, lrl3, with fewer lateral roots, and the differences in shoot height, primary root length and adventitious root length increased with the growth process compared to the wild-type plant. Auxin signaling pathway was also affected and became insensitive to gravity. The transgenic rice plants with over-expression of OsCYP2 were more tolerant to low temperature than the wild-type plants, suggesting that OsCYP2 was involved in the low temperature response in rice. In addition, OsCYP2 negatively regulated the expression of OsTPS38, a terpene synthase gene, and was dependent on the OsCDPK7-mediated pathway in response to low temperature stress. OsTPS38- overexpressed transgenic line ox-2 was more sensitive to low temperature. Therefore, OsCYP2 may negatively regulate OsTPS38 through an OsCDPK7-dependent pathway to mediate the response to low temperature in rice. These results provide a new basis for auxin signaling genes to regulate rice response to low temperature stress.

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Supplementary Material

Supplementary Material File

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