Open Access

ARTICLE

Proteomic Study of Differentially Expressed Proteins in Seeds between Parents and Offspring of Castor Bean (Ricinus communis L.)

Xiaotian Liang^1,#, Qi Wen^1,#, Rui Luo¹, Yanxin Zhang¹, Mingda Yin¹, Yanpeng Wen¹, Xuemei Hu¹, Zhiyan Wang¹, Yumiao Huo¹, Fenglan Huang^{1,2,3,4,5,6,*}

1 College of Life Science and Food Engineering, Inner Mongolia Minzu University, Tongliao, 028000, China
2 Key Laboratory of Castor Breeding of State Ethnic Affairs Commission, Tongliao, 028000, China
3 Research Center for Engineering and Technology of Castor Industry by Universities in Inner Mongolia Autonomous Region, Tongliao, 028000, China
4 Key Laboratory of Castor Breeding of Inner Mongolia Autonomous Region, Tongliao, 028000, China
5 Collaborative Innovation Center of Castor Industry of Inner Mongolia Autonomous Region, Tongliao, 028000, China
6 Engineering Research Center of Castor Industry Technology Innovation, Inner Mongolia Autonomous Region, Tongliao, 028000, China

* Corresponding Author: Fenglan Huang. Email:

(This article belongs to the Special Issue: Integrating Agronomy and Plant Physiology for Improving Crop Production)

Phyton-International Journal of Experimental Botany 2023, 92(6), 1765-1792. https://doi.org/10.32604/phyton.2023.026935

Received 10 October 2022; Accepted 27 December 2022; Issue published 11 April 2023

Abstract

Castor bean (Ricinus communis L.), is one of the top 10 oilseed crops in the world and, therefore, of high economic value. Hybridization is one of the most effective ways to breed new varieties with high yield, high oil content, and better stress resistance. Therefore, prediction of desired traits in castor hybrid offspring is particularly important. In this study, proteomic analysis was performed to identify differentially expressed proteins (DEPs) in seeds between castor hybrid offspring and their female (Lm female line aLmAB₂) and male parents (CSR·181). Among the DEPs upregulated in the seeds of hybrid offspring, the majority were related to seed yield and stress tolerance, while some were related to oil synthesis and fatty acid synthesis and metabolism in seeds. In other words, the hybrid offspring showed heterosis for seed yield, stress tolerance, oil synthesis, and fatty acid synthesis and metabolism when compared with their parents. Further, real-time quantitative polymerase chain reaction assays were performed on 12 genes encoding DEPs involved in oil synthesis, pollen abortion, yield, and stress tolerance of seeds. The results showed that the expression levels of the 12 genes were consistent with those of the DEPs.

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Keywords

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Supplementary Material

Supplementary Material File

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