Open Access

ARTICLE

Integrated Transcriptome and Small RNA Sequencing Analyses Reveals Insights into the Molecular Mechanism of Seed Germination in Mung Bean

Yanyan Pu^1,#, Liwen Wang^2,#, Leilei Li³, Yujun Si⁴, Shubin Xie², Yunzhe Cong¹, Dong Wang¹, Yongchao Gong¹, Rumei Tian¹, Xue Chen³, Xiaoyan Zhang¹, Min Liu¹, Hanfeng Ding^1,3,*, Nana Li^1,3,*

1 Institute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences, Jinan, 250100, China
2 State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, 271018, China
3 College of Life Science, Shandong Normal University, Jinan, 250014, China
4 Weifang Academy of Agricultural Sciences, Weifang, 261000, China

* Corresponding Authors: Hanfeng Ding. Email: ; Nana Li. Email:
# These authors contributed equally

(This article belongs to the Special Issue: Integrating Agronomy and Plant Physiology for Improving Crop Production)

Phyton-International Journal of Experimental Botany 2023, 92(6), 1793-1812. https://doi.org/10.32604/phyton.2023.026822

Received 27 September 2022; Accepted 03 January 2023; Issue published 11 April 2023

Abstract

During the life cycle of a plant, seed germination is crucial. Upon ingestion of water, the dry seeds resumed energy metabolism and cellular repair. To dissect the complex mechanisms at the very beginning of seed germination, two approaches including transcriptome and small RNA sequencing were conducted during the water imbibition process of mung bean seeds compared with dry seed. The transcriptome sequencing analysis identified 10,108 differentially expressed genes (DEGs) between dry and imbibed mung bean seeds. KEGG enrichment analyses demonstrated numerous DEGs involved in hormone signaling pathways, carbohydrate, and energy metabolism. Out of the total DEGs, 129 genes were investigated to involve in abscisic acid, gibberellin, and ethylene pathways, with 56 genes involved in glycolysis and TCA cycle. Through the analysis of small RNA sequencing among different samples, there were 284 miRNAs found in the imbibed and dried seeds of mung bean containing 213 known and 71 novel miRNAs. From comparison between the two types of seeds, 51 miRNAs were differentially expressed. The integrated analyses of transcriptome and miRNAome data showed that 23 DEGs are the putative target genes of 8 miRNAs including miR156, miR171b-3p, miR166e-3p, miR169-1, etc. QRT-PCR was used to validate several DEGs and miRNAs. The data suggested that the seed imbibition process is regulated by a variety of DEGs and microRNAs. Meanwhile, we hope to gain a deeper insight into how mung bean seeds germinate in order to optimize seed growth.

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Keywords

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Supplementary Material

Supplementary Material File

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