Open Access

ARTICLE

Comparative Non Participating Transcriptome Analysis Response to Low Phosphorus by CmPht1;2 in Chrysanthemum

by Chen Liu^1,2, Xiaowei Tang^1,2, Xiaohui Qu^1,2, Fadi Chen¹, Jiafu Jiang¹, Sumei Chen^1,*

1 State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Key Laboratory of Flower Biology and Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Nanjing, 210095, China
2 Nanjing Institute of Agricultural Sciences, Jiangsu Academy of Agricultural Sciences, Nanjing, 210046, China

* Corresponding Author: Sumei Chen. Email:

(This article belongs to the Special Issue: Identification of Genetic/Epigenetic Components Responding to Biotic and Abiotic Stresses in Crops)

Phyton-International Journal of Experimental Botany 2023, 92(5), 1391-1404. https://doi.org/10.32604/phyton.2023.027231

Received 20 October 2022; Accepted 28 November 2022; Issue published 09 March 2023

Abstract

Chrysanthemum morifolium Ramat is one of the four major cut flowers in the world. Pht1 family is focus on the uptake and transport of phosphate in plants. In our previous studies, CmPht1;2 overexpression line (Oe1) had higher phosphate contents both in roots and shoots, and its root development was significantly enhanced than wild type (WT) at low phosphorus conditions in chrysanthemum. Metabolomics analysis showed that several metabolites had a change in pyruvate metabolism and tricarboxylic acid (TCA) cycle pathway. To explore the gene difference expression and the change of metabolic pathway between CmPht1;2-Oe1 and WT, we conducted the transcriptome analysis. A total of 617,681 and 207,271 unigenes were obtained from roots and shoots, respectively. They were classified into biological process, cellular component and molecular function by Gene Ontology (GO). In addition, 450 different expression genes (DEGs) were found in the roots after 2 d treatment, and 1,787 DEGs were identified in shoots after 7 d treatment under LP condition between Oe1 and WT. From the top 20 pathways of DEGs assigned by Kyoto Encyclopedia of Genes and Genomes (KEGG), TCA cycle and pyruvate metabolism pathways mostly affected by overexpression of CmPht1;2 attracted our attention. This research will be helpful for elucidating the mechanism of effects by CmPht1;2 overexpression on growth, development and stress tolerance.

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Keywords

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Supplementary Material

Supplementary Material File

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