Open Access
ARTICLE
Cloning and Function Identification of a Phytoene Desaturase Gene from Eucommia ulmoides
Jiali Wang1, Xiangmei Chen1, Xiaozhen Huang1, Yichen Zhao1,*, Degang Zhao1,2,*
1 The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Tea Sciences, College of Life Sciences, Guizhou University, Guiyang, 550000, China
2 Plant Conservation Technology Center, Guizhou Key Laboratory of Agricultural Biotechnology, Guizhou Academy of Agricultural Sciences, Guiyang, 550000, China
* Corresponding Authors: Yichen Zhao. Email: ; Degang Zhao. Email:
(This article belongs to this Special Issue: Identification of Genetic/Epigenetic Components Responding to Biotic and Abiotic Stresses in Crops)
Phyton-International Journal of Experimental Botany 2023, 92(5), 1377-1389. https://doi.org/10.32604/phyton.2023.026830
Received 27 September 2022; Accepted 28 November 2022; Issue published 09 March 2023
Abstract
The phytoene desaturase (
PDS) encodes a crucial enzyme in the carotenoid biosynthesis pathway. Silencing or inhibiting
PDS expression leads to the appearance of mottled, chlorosis, or albino leaves. In this study, the CDS sequence of
EuPDS (
Eucommia ulmoides Phytoene Desaturase) was first cloned and then
PDS was silenced in
Nicotiana benthamiana. Result showed the expression level of
EuPDS in leaves was higher than that in the roots and stems. In
N. benthamiana leaves, which were treated by
Agrobacterium for 24 h, photo-bleaching was shown on the fresh leaves one week after injection and the transcript level of
PDS was down-regulated during the period of emersion. This suggested that
EuPDS could silence
PDS of
N. benthamiana, so as to cause the phenotype of leaf whitening.
PDS is the main reporter gene involved in virus-induced gene silencing (VIGS). This study offered molecular evidence for identifying
PDS gene involved in Carotenoid’s biosynthesis pathway and the regulation networks in
E. ulmides. It also laid a useful foundation for study on leaf discoloration mechanism of other woody plants.
Keywords
Cite This Article
Wang, J., Chen, X., Huang, X., Zhao, Y., Zhao, D. (2023). Cloning and Function Identification of a Phytoene Desaturase Gene from
Eucommia ulmoides.
Phyton-International Journal of Experimental Botany, 92(5), 1377–1389.