Pre-Breeding Genetic Diversity Assessment of Tomato (Solanum lycopersicum L.) Cultivars Based on Molecular, Morphological and Physicochemical Parameters
Jameel M. Al-Khayri1,*, Salha M. Alshamrani2, Adel A. Rezk1, Wael F. Shehata1, Mustafa I. Almaghasla3, Tarek A. Shalaby3,4, Ahmed M. Saad5, Fatmah A. Safhi6, Muhammad N. Sattar7, Arafat A. Abdel Latef8, Mahmoud Z. Sitohy5, Abdallah A. Hassanin9,*
1
Department of Agricultural Biotechnology, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa, 31982,
Saudi Arabia
2
Department of Biology, College of Science, University of Jeddah, Jeddah, 21959, Saudi Arabia
3
Department of Arid Land Agriculture, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa, 31982,
Saudi Arabia, Plant Pests, and Diseases Unit, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa, 31982,
Saudi Arabia
4
Department of Horticulture, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh, 33516, Egypt
5
Department of Biochemistry, Faculty of Agriculture, Zagazig University, Zagazig, 44511, Egypt
6
Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh, 11671,
Saudi Arabia
7
Central Laboratories, King Faisal University, Al-Ahsa, 31982, Saudi Arabia
8
Department of Botany and Microbiology, Faculty of Science, South Valley University, Qena, 83523, Egypt
9
Department of Genetics, Faculty of Agriculture, Zagazig University, Zagazig, 44511, Egypt
* Corresponding Authors: Jameel M. Al-Khayri. Email: ; Abdallah A. Hassanin. Email: ,
Phyton-International Journal of Experimental Botany 2023, 92(5), 1493-1512. https://doi.org/10.32604/phyton.2023.027375
Received 27 October 2022; Accepted 05 January 2023; Issue published 09 March 2023
Abstract
Appropriate knowledge of the parental cultivars is a pre-requisite for a successful breeding program. This study
characterized fruit yield, quality attributes, and molecular variations of ten tomato cultivars during three consecutive generations under greenhouse conditions. Peto 86, Castle Rock, and Red Star cultivars showed the highest
fruit yield (kg/plant), total phenolic compounds (TPC), and sap acidity. Principal component analysis categorized
the evaluated fruit yield into three groups based on their quality attributes. A robust positive correlation appeared
among traits inside each group. A positive correlation was likewise noticed between the first and the second
groups. However, a negative correlation was detected between the first, the second and the third group. Molecular
profiling, using seven inter-simple sequence repeat (ISSR) primers, produced 60 loci, including 49 polymorphic
loci. The molecular analysis also pinpointed the highest genetic similarity (0.92) between P73 and Moneymaker,
while the lowest genetic similarity (0.46) was observed between Castle Rock and Moneymaker. The cultivars
P73 and Moneymaker showed the lowest genetic distance (2.24), while the highest genetic distance (5.92) was
observed between Super Marmand and Peto86, on the one hand, and between Castle Rock and Moneymaker,
on the other hand. The chemical analysis of fruit sap indicated the highest levels of TPC, total flavonoids, anthocyanin, ascorbic acid and total soluble solids in Peto 86 and Castle Rock cultivars. Phylogeny analysis of tomato
cultivars based on morphological and molecular attributes indicated four distinct clades. Peto 86, Castle Rock, and Red star cultivars can be recommended for the tomato hybridization breeding programs in the future, with other
tomato cultivars as potentially high-yielding parents.
Keywords
Cite This Article
APA Style
Al-Khayri, J.M., Alshamrani, S.M., Rezk, A.A., Shehata, W.F., Almaghasla, M.I. et al. (2023). Pre-breeding genetic diversity assessment of tomato (solanum lycopersicum L.) cultivars based on molecular, morphological and physicochemical parameters. Phyton-International Journal of Experimental Botany, 92(5), 1493-1512. https://doi.org/10.32604/phyton.2023.027375
Vancouver Style
Al-Khayri JM, Alshamrani SM, Rezk AA, Shehata WF, Almaghasla MI, Shalaby TA, et al. Pre-breeding genetic diversity assessment of tomato (solanum lycopersicum L.) cultivars based on molecular, morphological and physicochemical parameters. Phyton-Int J Exp Bot. 2023;92(5):1493-1512 https://doi.org/10.32604/phyton.2023.027375
IEEE Style
J.M. Al-Khayri et al., "Pre-Breeding Genetic Diversity Assessment of Tomato (Solanum lycopersicum L.) Cultivars Based on Molecular, Morphological and Physicochemical Parameters," Phyton-Int. J. Exp. Bot., vol. 92, no. 5, pp. 1493-1512. 2023. https://doi.org/10.32604/phyton.2023.027375