Open Access

ARTICLE

Genetic Diversity and Population Structure Analysis of Barley Landraces from Shanghai Region Using Genotyping-by-Sequencing

by Luli Li^1,2, Nigel G. Halford³, Huihui Wang⁴, Yingjie Zong¹, Zhenzhu Guo¹, Ruiju Lu¹, Chenghong Liu¹, Zhiwei Chen^1,*

1 Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, China
2 College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China
3 Rothamsted Research, Harpenden, AL5 2JQ, UK
4 Qingdao OE Biotechnology Company Limited, Qingdao, 266100, China

* Corresponding Author: Zhiwei Chen. Email:

(This article belongs to the Special Issue: Identification of Genetic/Epigenetic Components Responding to Biotic and Abiotic Stresses in Crops)

Phyton-International Journal of Experimental Botany 2023, 92(4), 1275-1287. https://doi.org/10.32604/phyton.2023.026946

Received 05 October 2022; Accepted 11 November 2022; Issue published 06 January 2023

Abstract

Barley (Hordeum vulgare L.) is an important economic crop for food, feed and industrial raw materials. In the present research, 112 barley landraces from the Shanghai region were genotyped using genotyping-by-sequencing (GBS), and the genetic diversity and population structure were analyzed. The results showed that 210,268 Single Nucleotide Polymorphisms (SNPs) were present in total, and the average poly-morphism information content (PIC) was 0.1642. Genetic diversity and population structure analyses suggested that these barley landraces were differentiated and could be divided into three sub-groups, with morphological traits of row-type and adherence of the hulls the main distinguishing factors between groups. Genotypes with similar or duplicated names were also investigated according to their genetic backgrounds and seed appearances. This study provided valuable information on barley landraces from the Shanghai region, and showed that all these barley landraces should be protected and used for future breeding programs.

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Keywords

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Supplementary Material

Supplementary Material File

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