Open Access iconOpen Access

ARTICLE

crossmark

VvAGAMOUS Affect Development of Four Different Grape Species Ovary

Pengfei Zhang1,#, Yuqin Zhang1,#, Qifeng Zhao2, Tiequan Niu1, Pengfei Wen1,*, Jinjun Liang1,*

1 College of Horticulture, Shanxi Agricultural University, Taigu, 030801, China
2 Pomology Institute, Shanxi Agricultural University, Shanxi Academy of Agricultural Sciences, Taigu, 030801, China

* Corresponding Authors: Pengfei Wen. Email: email; Jinjun Liang. Email: email

(This article belongs to this Special Issue: Identification of Genetic/Epigenetic Components Responding to Biotic and Abiotic Stresses in Crops)

Phyton-International Journal of Experimental Botany 2023, 92(4), 1125-1138. https://doi.org/10.32604/phyton.2023.026227

Abstract

Grape pistil has an important influence on fruit size and quality. However, there were few studies on grape ovary, and the development process of the ovary is still unclear. Therefore, in this paper, four different grape varieties with different lengths of small inflorescences, namely ‘Musct Hambourg’ grape (Vitis vinifera), ‘Concord’ grape (Vitis labrusca), ‘ShanPuTao’ grape (Vitis amurensis) and ‘GongNiang2Hao’ grape (Vitis amurensis × Vitis vinifera) were used as test materials. Four varieties ovary were significant differences by means of stereomicroscope, paraffin section. The expression of ovary determining gene VvAGAMOUS (VvAG) and its development related genes VvCRABS CLAW (VvCRC) and VvAGAMOUS-LIKE 11  (VvAGL11) with similar functions during the development of different grape varieties were preliminarily explored using fluorescence quantitative test. The relationship between VvAG and VvCRC, VvAG and VvAGL11 were analyzed using Y1H assay. Our results showed that there were obvious abdominal sutures on the surface of expect for ‘Musct Hambourg’ grape, and existing poly carpels. The ovary development of ‘ShanPuTao’ and ‘GongNiang2Hao’ grape was completed when the inflorescence length was less than 1 cm, while the ‘Concord’ and ‘Musct Hambourg’ grape were fully developed when the length of inflorescence was 3–4 and 4–5 cm, respectively. VvAG and VvCRC began to express in large quantities after the formation of stamen primordia, while VvAGL11 during the forming of ovule primordia. Therefore, VvAG and VvCRC mainly regulated the development of stamens and carpels and also promote the development of ovules, while VvAGL11 major regulated the development of ovules. The promoters of VvCRC and VvAGL11 were bound by VvAG. This study provides an important theoretical basis for further research on the molecular mechanism of grape ovary development.

Keywords


Cite This Article

Zhang, P., Zhang, Y., Zhao, Q., Niu, T., Wen, P. et al. (2023). VvAGAMOUS Affect Development of Four Different Grape Species Ovary. Phyton-International Journal of Experimental Botany, 92(4), 1125–1138.



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 774

    View

  • 675

    Download

  • 1

    Like

Share Link