Open Access

ARTICLE

The Improvement of Soybean Salt Tolerance by Overexpressed GmPAO1

by Yeyao Du, Yang Song, Ye Zhang, Sujie Fan, Hanzhu Zhang, Piwu Wang^*

College of Agronomy, Jilin Agricultural University, Changchun, 130118, China

* Corresponding Author: Piwu Wang. Email:

(This article belongs to the Special Issue: Abiotic and Biotic Stress Tolerance in Crop)

Phyton-International Journal of Experimental Botany 2023, 92(4), 1109-1124. https://doi.org/10.32604/phyton.2023.025503

Received 17 July 2022; Accepted 17 October 2022; Issue published 06 January 2023

Abstract

Polyamines play an important regulatory role during plant growth and development and adversity stress, and polyamine oxidase (PAO) is involved in polyamine catabolism. In this study, an up-regulated polyamine oxidase gene GmPAO1 was obtained by transcriptome sequencing analysis and screening at soybean seedling stages. Also, its expression pattern and function were analyzed. The identification results of transgenic GmPAO1 soybean positive lines showed that the relative expression level of GmPAO1 in the overexpressed lines was increased under salt stress. With increasing stress concentration, the seed germination rate decreased. However, the seed germination rate of the overexpressed lines was significantly higher than that of the control lines, and the phenotypic character of the root systems was also better than that of the control lines. The measurement of superoxide dismutase (SOD) and peroxidase (POD) activities and malondialdehyde and hydrogen peroxide contents revealed that the overexpressed soybean lines significantly increased the SOD and POD activities, significantly reducing the malondialdehyde content. Although the hydrogen peroxide content in the transformed plants gradually increased, the hydrogen peroxide content in the overexpression lines was still lower than that in the gene editing lines. Based on this, it was preliminarily judged that GmPAO1 can improve soybean salt tolerance.

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Keywords

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