Open Access
ARTICLE
A Novel Dominant Allele from 93-11, ES(4), Represses Reactive Oxygen Species Scavenging and Leads to Early-Senescence in Rice
Zhishu Jiang#, Cong Gan#, Yulian Liu, Xiaoli Lin, Limei Peng, Yongping Song, Xiaowei Luo, Jie Xu*
Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, 330045, China
* Corresponding Author: Jie Xu. Email:
(This article belongs to the Special Issue: High-Yield Rice Physiology & Genetics)
Phyton-International Journal of Experimental Botany 2023, 92(3), 665-677. https://doi.org/10.32604/phyton.2023.025266
Received 02 July 2022; Accepted 25 August 2022; Issue published 29 November 2022
Abstract
Senescence is the last developmental process in plant, which has an important impact on crop yield and quality. In this study, a stable hereditary early-senescence line BC64 was isolated from the high-generation recombinant inbred lines of 93-11 and Wuyunjing7 (W7). Genetic analysis showed that the premature aging phenotype was controlled by a dominant gene derived from 93-11. By linkage analysis, the gene was primarily mapped in the region between marker B4 and B5 near the centromere of chromosome 4, described as
ES(4). Through multiple backcrossing with W7, the near-isogenic line of
ES(4) (NIL-ES(4)) was obtained. Compared with wild-type W7, NIL-ES(4) plants showed more sever senescence phenotype in both nature and dark conditions. In NIL plants, leaves turned yellow at the fully tillering stage; photosynthetic rate, pollen fertility and seed setting rate were decreased. Moreover, the malondialdehyde, proline content and relative conductivity in NIL-ES(4) were significantly higher than those in W7; both transcript level and activities of reactive oxygen species scavenging enzymes were repressed; H
2O
2 and O
2− were significantly accumulated. This study provides a basis for further cloning and function identification of
ES(4).
Keywords
Cite This Article
Jiang, Z., Gan, C., Liu, Y., Lin, X., Peng, L. et al. (2023). A Novel Dominant Allele from 93-11,
ES(4), Represses Reactive Oxygen Species Scavenging and Leads to Early-Senescence in Rice.
Phyton-International Journal of Experimental Botany, 92(3), 665–677. https://doi.org/10.32604/phyton.2023.025266