Global and Comparative Proteome Analysis of Nitrogen-Stress Responsive Proteins in the Root, Stem and Leaf of <i>Brassica napus</i>

Liang Chai; Cheng Cui; Benchuan Zheng; Jinfang Zhang; Jun Jiang; Haojie Li; Liangcai Jiang

doi:10.32604/phyton.2023.024717

Table of Content

Open Access

ARTICLE

Global and Comparative Proteome Analysis of Nitrogen-Stress Responsive Proteins in the Root, Stem and Leaf of Brassica napus

Liang Chai^1,2, Cheng Cui¹, Benchuan Zheng¹, Jinfang Zhang¹, Jun Jiang¹, Haojie Li^1,2,*, Liangcai Jiang^1,*
1 Crop Science Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China
2 Environment-Friendly Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province, Chengdu, 610066, China
* Corresponding Authors: Haojie Li. Email: ; Liangcai Jiang. Email:
(This article belongs to this Special Issue: Plant Omics in Challenging Environment)

Phyton-International Journal of Experimental Botany 2023, 92(3), 645-663. https://doi.org/10.32604/phyton.2023.024717

Received 06 June 2022; Accepted 29 August 2022; Issue published 29 November 2022

Abstract

Nitrogen (N) is one of the basic nutrients and signals for plant development and deficiency of it would always limit the productions of crops in the field. Quantitative research on expression of N-stress responsive proteins on a proteome level remains elusive. In order to gain a deep insight into the proteins responding to nitrogen stress in rapeseed (Brassica napus L.), comparative proteomic analysis was performed to investigate changes of protein expression profiles from the root, stem and leaf under different N concentrations, respectively. More than 200 differential abundance proteins (DAPs) were detected and categorized into groups according to annotations, including “binding and catalytic activity”, “involved in primary metabolism and cellular processes”, “stress-response” and so on. Variation in chlorophyll (Chl) content and antioxidant activities further revealed that oxidative stress raised with the increase of N concentration. Bioinformatics analysis based on the expression level of total proteins suggested these DAPs might play important roles in adaptation to N-stress conditions. Generally, these results provides a new aspect into N-stress responding proteins in Brassica plants.

Keywords

Brassica napus; nitrogen (N) response; oxidative stress; proteomics

Supplementary Material

Supplementary Material File

Cite This Article

Chai, L., Cui, C., Zheng, B., Zhang, J., Jiang, J. et al. (2023). Global and Comparative Proteome Analysis of Nitrogen-Stress Responsive Proteins in the Root, Stem and Leaf of Brassica napus. Phyton-International Journal of Experimental Botany, 92(3), 645–663.

This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.