Open Access
ARTICLE
Genome-Wide Identification, Evolution and Expression Analyses of GA2ox Gene Family in Brassica napus L.
Yanhua Li1,#, Hualei Huang1,#, Youming Shi1, Shuqin Huang1, Tao Liu1, Changming Xiao1, Xiaoqing Tian2, Ping Zhao1, Xiaoyan Dai3, Taocui Huang1,*, Yan Zhou1,*
1 Chongqing Academy of Agricultural Sciences, Chongqing, 401329, China
2 Chongqing Municipal Agricultural School, Chongqing, 401329, China
3 Fengdu Agricultural Technology Service Center, Chongqing, 408201, China
* Corresponding Authors: Taocui Huang. Email: ; Yan Zhou. Email:
(This article belongs to this Special Issue: Identification of Genetic/Epigenetic Components Responding to Biotic and Abiotic Stresses in Crops)
Phyton-International Journal of Experimental Botany 2023, 92(3), 815-835. https://doi.org/10.32604/phyton.2023.023928
Received 18 May 2022; Accepted 09 September 2022; Issue published 29 November 2022
Abstract
Gibberellin 2-oxidases (
GA2ox) are important enzymes that maintain the balance of bioactive GAs in plants.
GA2ox genes have been identified and characterized in many plants, but these genes were not investigated in
Brassica napus. Here, we identified 31
GA2ox genes in
B. napus and 15 of these
BnaGA2ox genes were distributed in the A and C subgenomes. Subcellular localization predictions suggested that all
BnaGA2ox proteins were localized in the cytoplasm, and gene structure analysis showed that the
BnaGA2ox genes contained 2–4 exons. Phylogenetic analysis indicated that
BnGA2ox family proteins in monocotyledons and dicotyledons can be divided into four groups, including two C
19-GA2ox and two C
20-GA2ox clades. Group 4 is a C
20-GA2ox Class discovered recently. Most
BnaGA2ox genes had a syntenic relationship with
AtGA2ox genes.
BnaGA2ox genes in the C subgenome had experienced stronger selection pressure than genes in the A subgenome.
BnaGA2ox genes were highly expressed in specific tissues such as those involved in growth and development, and most of them were mainly involved in abiotic responses, regulation of phytohormones and growth and development. Our study provided a valuable evolutionary analysis of
GA2ox genes in monocotyledons and dicotyledons, as well as an insight into the biological functions of
GA2ox family genes in
B. napus.
Keywords
Cite This Article
Li, Y., Huang, H., Shi, Y., Huang, S., Liu, T. et al. (2023). Genome-Wide Identification, Evolution and Expression Analyses of
GA2ox Gene Family in
Brassica napus L..
Phyton-International Journal of Experimental Botany, 92(3), 815–835.