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Construction of EMS-Induced Peanut Mutant Libraries and Identification of Pod-Related Traits Mutant Lines

Hao Chen1, Faqian Xiong2,*, Rilong Xu1, Xiangyu Chen1, Haifeng Zhong1, Yumei Zhang1, Xinlong Lan1, Hong Zhang1, Yuhua Chen1, Runfang Hu1, Guoqiang Lin1,*, Zhaoxiu Tang1
1 Institute of Crop Sciences, Fujian Academy of Agricultural Sciences, Fujian Research Station of Crop Gene Resource & Germplasm Enhancement, Ministry of Agriculture and Rural Affairs of People’s Republic of China, Fujian Engineering Research Center for Characteristic Upland Crops Breeding, Fujian Engineering Laboratory of Crop Molecular Breeding, Fuzhou, 350013, China
2 Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007, China
* Corresponding Authors: Faqian Xiong. Email: ; Guoqiang Lin. Email:
(This article belongs to this Special Issue: Gene Function and Evolution in Arachis)

Phyton-International Journal of Experimental Botany 2023, 92(2), 537-557.

Received 15 May 2022; Accepted 22 June 2022; Issue published 12 October 2022


Peanut (Arachis hypogaea L.) is an oil and economic crop of vital importance, and peanut pod is the key organ influencing the yield and processing quality. Hence, the Pod-related traits (PRTs) are considered as important agronomic traits in peanut breeding. To broaden the variability of PRTs in current peanut germplasms, three elite peanut cultivars were used to construct Ethyl methane sulfonate (EMS)-induced mutant libraries in this study. The optimal EMS treatment conditions for the three peanut varieties were determined. It was found that the median lethal dose (LD50) of EMS treatment varied greatly among different genotypes. Finally, the EMS-induced peanut mutant libraries were constructed and a total of 124 mutant lines for PRTs were identified and evaluated. Furthermore, “M-8070”, one of the mutant lines for pod constriction, was re-sequenced via high-throughput sequencing technology. The genome-wide variations between “M-8070” and its wild parent “Fuhua 8” (FH 8) were detected. 2994 EMS-induced single nucleotide polymorphisms (SNPs) and 1188 insertion-deletions (InDels) between “M-8070” and its wild parent were identified. The predominant SNP mutation type was C/G to T/A transitions, while the predominant InDel mutation type was “1-bp”. We analyzed the distribution of identified mutations and annotated their functions. Most of the mutations (91.68% of the SNPs and 77.69% of the InDels) were located in the intergenic region. 72 SNPs were identified in the exonic region, leading to 27 synonymous, 43 non-synonymous and 2 stop-gain variation for gene structure. 13 Indels were identified in the exonic region, leading to 4 frame-shift, 8 non-frame-shift and 1 stop-gain variations of genes. These mutations may lead to the phenotypic variation of “M-8070”. Our study provided valuable resources for peanut improvement and functional genomic research.


Peanut; EMS-induced; mutant line; pod-related traits; re-sequencing; pod constriction

Cite This Article

Chen, H., Xiong, F., Xu, R., Chen, X., Zhong, H. et al. (2023). Construction of EMS-Induced Peanut Mutant Libraries and Identification of Pod-Related Traits Mutant Lines. Phyton-International Journal of Experimental Botany, 92(2), 537–557.

This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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