Open Access

ARTICLE

A Rapid Determination of 30 Prohibited Pesticide Residues in Lycii Fructus by UPLC-MS/MS

by Kesheng Lin^1,3,#, Zhengqi Wang^2,#, Li Wang^1,3, Jiawen Zhou^1,3, Lijuan Han^1,3, Jingjing Wen², Tingxia Dong², Ran Duan^2,*, Ning Li^1,3,*

1 KMHD, Shenzhen, 518128, China
2 Shenzhen Key Laboratory of Edible and Medicinal Bioresources, HKUST Shenzhen Research Institute, Shenzhen, 518057, China
3 Kangmei Pharmaceutical Co., Ltd., Jieyang, 515300, China

* Corresponding Authors: Ran Duan. Email: ; Ning Li. Email:
# These two authors contributed equally to this work

Phyton-International Journal of Experimental Botany 2023, 92(2), 423-437. https://doi.org/10.32604/phyton.2022.023763

Received 13 May 2022; Accepted 06 June 2022; Issue published 12 October 2022

Abstract

Prohibited pesticide residues have become one of the main factors affecting the quality and safety of Lycii Fructus, However, rarely studies focus on the rapid determination of these residues. Here, a total of 30 kinds of prohibited pesticide residues were determined by ultra-performance liquid chromatography tandem triple quadrupole mass spectrometry (UPLC-MS/MS) in five different process ways. Pretreatment methods, chromatographic separation and detection conditions in mass spectrometry were all optimized accordingly. Among the five different pretreatment methods, the first and third solid phase extraction failed to provide high recoveries of sulfosulfuron compounds (both lower than 60%). Recovery of chlorphenamidine by the Quick Easy Cheap Effective Rugged and Safe multiresidue method (QuEChERS) was lower than 60%, which did not meet the requirements of trace determination. The concentrations of 30 prohibited pesticides residues treated by straightforward and solid phase extraction showed good linearity in their corresponding ranges, with correlation coefficients over 0.99. The average recoveries of straightforward ranged from 78.13% to 110.9%, while RSD ranged from 1.3% to 16.9%, albeit poor purification was observed. The recovery yield from solid phase extraction was between 67.75% and 103.08% with RSD value from 0.8% to 14.0%, which met the requirements of trace determination, this method has good precision and stability. These results could be employed to other Traditional Chinese Medicines (TCMs) in detecting prohibited pesticide residues.

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Keywords

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