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Functional Analysis of the Genotypic Differences in Response of Pea (Pisum sativum L.) to Calcareous-Induced Iron Deficiency

Sameh Barhoumi1, Hasna Ellouzi2, Abdelmajid Krouma1,3,*
1 Laboratory of Ecosystems and Biodiversity in Arid Land of Tunisia, Faculty of Sciences, University of Sfax, Sfax, 3000, Tunisia
2 Centre of Biotechnology of Borj Cedria, Hammam Lif, 2050, Tunisia
3 Faculty of Sciences and Techniques, University of Kairouan, Kairouan, 9100, Tunisia
* Corresponding Author: Abdelmajid Krouma. Email:
(This article belongs to this Special Issue: Plant Ecophysiology: Recent Trends and Advancements)

Phyton-International Journal of Experimental Botany 2023, 92(2), 521-536. https://doi.org/10.32604/phyton.2022.023555

Received 01 May 2022; Accepted 16 June 2022; Issue published 12 October 2022

Abstract

Lime-induced iron chlorosis is a major nutritional disorder causing severe plant growth and yield reduction in the calcareous soils of Tunisia. The understanding the behavior of key metabolic functions of peas on calcareous soils, the identification of useful traits of tolerance, and the exploration of the genotypic differences in response to this constraint remain the most efficient approaches due to their coast, environmental benefits, and sustainability. For this purpose, a greenhouse experiment was conducted on three pea genotypes (Alexandra: Alex, Douce de provence: DP, and Merveille de Kelvedon: MK) cultivated on calcareous soil (Fe-deficient) and fertile soil (control). Plant growth, SPAD index, iron nutrition and distribution, photosynthesis, and antioxidant enzymes were deeply analyzed to discriminate genotypic differences. Calcareous-induced iron deficiency reduced SPAD index, plant growth, net photosynthesis, and tissue Fe content against a significant stimulation of the oxidative stress indicators, H2O2 and Malondialdehyde (MDA). Moreover, we reported a significant induction of SOD and CAT activity in shoots and roots of the Alexandra genotype. Fe use efficiency increased on calcareous soil and clearly discriminated the studied genotypes. Alexandra genotype was found to be the most tolerant to lime-induced iron chlorosis. This genotype protects its tissues against oxidative stress by stimulating enzyme activities (SOD and CAT) and develops significant efficiency of Fe uptake, translocation to shoots and use when cultivated on calcareous soil.

Keywords

Calcareous-induced Fe deficiency; catalase; Fe use efficiency; photosynthesis; superoxide dismutase

Cite This Article

Barhoumi, S., Ellouzi, H., Krouma, A. (2023). Functional Analysis of the Genotypic Differences in Response of Pea (Pisum sativum L.) to Calcareous-Induced Iron Deficiency. Phyton-International Journal of Experimental Botany, 92(2), 521–536.



This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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