TY - EJOU
AU - Huang, Ruizhi
AU - Yu, Huasheng
AU - Yang, Yong
AU - Liu, Heqin
AU - Wu, Xuelong
AU - Liu, Zhihong
AU - He, Haiyan
AU - Wu, Gengwei
AU - Wang, Wengjia
AU - Wang, Hua
TI - Effects of Heat Stress during Seed Filling Stage on Brassica napus Seed Oil Accumulation and Chlorophyll Fluorescence Characteristics
T2 - Phyton-International Journal of Experimental Botany
PY - 2023
VL - 92
IS - 2
SN - 1851-5657
AB - As global temperature rise, the threat of heat stress to rapeseed production is becoming more obvious. Exploring the response characteristics of two important biological pathways, oil accumulation and photosynthesis, to heat stress during B. napus seed filling is helpful in the genetic improvement of heat-tolerant rapeseed. The effects of heat stress on seed oil accumulation and chlorophyll fluorescence characteristics of 29 B. napus germplasms with different oil content and environmental sensitivity, including 6 rapeseed varieties which exhibited environment-sensitive/insensitive and with high, medium or low oil content, were tested by whole plant heat stress or the in vitro silique culture system. Both assay exhibited similar trend on oil content of the rapeseed germplasms. The heat effect on the chlorophyll fluorescence kinetic parameters Fv/Fm, ETR and Y(II) were also consistent. Heat stress significantly decreased oil content, although there was abundant genetic variation on heat tolerance among the genotypes. Correlation analysis showed that the decrease rate of Fv/Fm of silique heat-stressed B. napus developing seed was positive correlative to the decrease rate of mature seed oil content of the whole plant heat-stressed rapeseed (R = 0.9214, P-value < 0.01). Overall, the results indicated that heat stress inhibited oil accumulation and photosynthesis in B. napus developing seed. The decrease rate of chlorophyll fluorescence parameter Fv/Fm of heat-stressed developing seed could be used as the index of heat tolerant rapeseed identification. Further, two heat insensitive rapeseed varieties with high oil content were identified.
KW - Brassica napus L.; heat stress; seed filling stage; oil accumulation; chlorophyll fluorescence characteristics
DO - 10.32604/phyton.2022.023252