Open Access

ARTICLE

Arbuscular Mycorrhizal Fungi Alleviates Salt-Alkali Stress Demage on Syneilesis aconitifolia

Linlin Fang, Jiamei Xu, Chunxue Yang^*

College of Landscape Architecture, Northeast Forestry University, Harbin, 150040, China

* Corresponding Author: Chunxue Yang. Email:

Phyton-International Journal of Experimental Botany 2023, 92(12), 3195-3209. https://doi.org/10.32604/phyton.2023.043049

Received 20 June 2023; Accepted 07 September 2023; Issue published 28 December 2023

Abstract

Syneilesis aconitifolia is a potential ground cover and decorative material in gardens, which exhibits a strong salt-alkali tolerance, and also has medicinal value. In this study, the arbuscular mycorrhizal (AM) fungi community in the soil surrounding S. aconitifolia roots in the Songnen saline-alkali grassland was used as the inoculation medium for a pot cultivation experiment. After normal culture for 90 days, NaCl and NaHCO₃ solutions were applied to subject plants to salt or alkali stress. Solution concentrations of 50, 100, and 200 mmol/L were applied for 10 days, and mycorrhizal colonization, biomass, relative water content (RWC), chlorophyll concentration, malondialdehyde (MDA) concentration, antioxidant system activity, and osmomodulator concentration were determined to identify the effects of AM fungi on root colonization status and salinity tolerance in S. aconitifolia. There were three key results. (1) Compared to the controls, the intensity and rate of colonization decreased under saline-alkali stress, and the adaptability of AM fungi under low concentration alkali stress was higher than that under salt stress. (2) The AM fungi could increase the biomass, RWC, and chlorophyll concentration, and decrease the MDA concentration of S. aconitifolia to some extent. With an increase in the salt or alkali solution concentration, AM fungi not only upregulated the activity of the antioxidant system, but also increased the concentration of osmotic regulatory substances. (3) A multivariate analysis of variance (ANOVA) and radar map analysis showed that the mechanisms of resistance to salt and alkali stress were not the same in S. aconitifolia. In the salt treatment, AM fungi mainly regulated salt stress through osmotic regulatory substances such as soluble sugars, soluble proteins, and proline. In the alkali treatment, AM fungi mainly regulated alkali stress through glutathione (GSH), soluble sugars, and MDA. The results showed that the colonization rate of S. aconitifolia under low concentration alkali stress was higher than that under salt stress, and the inoculation of AM fungi could significantly reduce the MDA concentration of S. aconitifolia plants under salinity and alkali stress, and improve the antioxidant enzyme activity and osmoregulatory substance accumulation, thereby improving the salinity tolerance of S. aconitifolia.

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Keywords

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Supplementary Material

Supplementary Material File

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