Open Access

ARTICLE

Transcriptome Analysis of Molecular Mechanisms Underlying Phenotypic Variation in Phaseolus vulgaris Mutant ‘nts’

Limin Yin^#, Chang Liu^#, Zicong Liang, Dajun Liu, Guojun Feng, Zhishan Yan^*, Xiaoxu Yang^*

Horticulture Department, College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin, China

* Corresponding Authors: Zhishan Yan. Email: ; Xiaoxu Yang. Email:

Phyton-International Journal of Experimental Botany 2023, 92(11), 2981-2998. https://doi.org/10.32604/phyton.2023.043151

Received 23 June 2023; Accepted 07 August 2023; Issue published 24 October 2023

Abstract

The phenotype of a common bean plant is often closely related to its yield, and the yield of plants with reduced height or poor stem development during growth is low. Mutants serve as an essential gene resource for common bean breeding genetic research. Although model plants and crops are studied to comprehend the molecular mechanisms and genetic basis of plant phenotypes, the molecular mechanism of phenotypic variation in common beans remains underexplored. We here used the mutant ‘nts’ as material for transcriptome sequencing analysis. This mutant was obtained through ⁶⁰Co-γ irradiation from the common bean variety ‘A18’. Differentially expressed genes were mainly enriched in GO functional entries such as cell wall organization, auxin response and transcription factor activity. Metabolic pathways significantly enriched in KEGG analysis included plant hormone signal transduction pathways, phenylpropanoid biosynthesis pathways, and fructose and mannose metabolic pathways. AUX1 (Phvul.001G241500), the gene responsible for auxin transport, may be the key gene for auxin content inhibition. In the plant hormone signal transduction pathway, AUX1 expression was downregulated and auxin transport across the membrane was blocked, resulting in stunted growth of the mutant ‘nts’. The results provide important clues for revealing the molecular mechanism of ‘nts’ phenotype regulation in bean mutants and offer basic materials for breeding beneficial phenotypes of bean varieties.

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Keywords

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