Open Access
ARTICLE
Ectopic Overexpression of EuCHIT30.7 Improves Nicotiana tabacum Resistance to Powdery Mildew
Lanjun Li1, Degang Zhao1,2,*
1 Key Laboratory of Mountain Plant Resources Protection and Germplasm Innovation of the Ministry of Education, College of Life Sciences/Institute of Agricultural Bioengineering, Guizhou University, Guiyang, 550025, China
2 Plant Conservation Technology Center, Guizhou Key Laboratory of Agricultural Biotechnology, Biotechnology Institute of Guizhou, Guizhou Academy of Agricultural Sciences, Guiyang, 550006, China
* Corresponding Author: Degang Zhao. Email:
Phyton-International Journal of Experimental Botany 2023, 92(11), 3043-3061. https://doi.org/10.32604/phyton.2023.031175
Received 18 May 2023; Accepted 08 August 2023; Issue published 24 October 2023
Abstract
Various strains of powdery mildew (PM), a notorious plant fungal disease, are prevalent and pose a significant threat to plant health. To control PM, transgenic technology can be used to cultivate more resistant plant varieties. In the present study, we utilized the rapid amplification of cDNA ends (RACE) technique to clone the full-length cDNA sequence of the
EuCHIT30.7 gene to explore plant genes with disease resistance functions. Bioinformatics analysis revealed that this gene belongs to the GH18 family and is classified as a class III chitinase. The
EuCHIT30.7 gene is expressed throughout the
Eucommia ulmoides plant, with the most abundant expression in male flowers. Subcellular localization analysis indicated that the protein encoded by this gene was detected within both the cell membrane and cytoplasm. Upon PM inoculation, overexpression of
EuCHIT30.7 in tobacco plants led to a significantly reduced relative lesion area and a decreased spore count compared to both wild-type and empty vector control plants. Activities of the protective enzymes, namely, peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), and phenylalaninammo-nialyase (PAL), in tobacco plants overexpressing
EuCHIT30.7 were significantly greater than those in wild-type and empty vector tobacco plants. Furthermore, the rate of increase in malondialdehyde (MDA) content was significantly lower in tobacco plants expressing
EuCHIT30.7 compared to control tobacco plants. In
EuCHIT30.7 transgenic tobacco, the expression of pathogen-related protein genes, namely,
PR2,
PR5,
PR1a,
PDF1.2, and
MLP423, along with the tobacco PM negative regulatory gene,
MLO2, were significantly higher compared to control tobacco plants. These findings suggested that
EuCHIT30.7 significantly enhances the resistance of tobacco to PM.
Keywords
Cite This Article
APA Style
Li, L., Zhao, D. (2023). Ectopic overexpression of euchit30.7 improves nicotiana tabacum resistance to powdery mildew. Phyton-International Journal of Experimental Botany, 92(11), 3043-3061. https://doi.org/10.32604/phyton.2023.031175
Vancouver Style
Li L, Zhao D. Ectopic overexpression of euchit30.7 improves nicotiana tabacum resistance to powdery mildew. Phyton-Int J Exp Bot. 2023;92(11):3043-3061 https://doi.org/10.32604/phyton.2023.031175
IEEE Style
L. Li and D. Zhao, "Ectopic Overexpression of EuCHIT30.7 Improves Nicotiana tabacum Resistance to Powdery Mildew," Phyton-Int. J. Exp. Bot., vol. 92, no. 11, pp. 3043-3061. 2023. https://doi.org/10.32604/phyton.2023.031175