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Extraction, Antioxidant Activity and Identification of Flavonoids from Root Tubers of Kosteletzkya virginica

Yu Zai*, Shaohua Liu
School of Life Sciences and Chemical Engineering, Jiangsu Second Normal University, Nanjing, 210013, China
* Corresponding Author: Yu Zai. Email:
(This article belongs to this Special Issue: Plant Bioactive Compounds-Chemotaxonomic Significance, Antioxidant Properties, Potential Application as Food Ingredients as well as Therapeutics, and the Analytical Technology in Their Efficient Discovery)

Phyton-International Journal of Experimental Botany 2023, 92(1), 225-236.

Received 16 March 2022; Accepted 17 May 2022; Issue published 06 September 2022


Kosteletzkya virginica (K. virginica) is used for revegetation of salt-affected coastal tidal flats and as a raw material of biodiesel. K. virginica root tuber, a biowaste with low economic value, is rich in bioactive compounds. This study aimed to extract and identify flavonoids from K. virginica root tubers. The optimal extraction conditions were 1/25 (w/v) solid/liquid ratio, 40% ethanol concentration at 40°C for 60 min. Under these conditions, 65.2 ± 3.7 mg/g total flavonoid content was extracted from the roots, which were collected from salinized soil in late autumn of the third year. Antioxidant activity was evaluated through 1,1-diphenyl-2-picrylhydrazyl, hydroxyl radical, and superoxide anion scavenging assays. The extracted flavonoids exhibited antioxidant activity in a dose-dependent manner. Five flavonoids, glucoliquiritin apioside, licoisoflavone B, 5-methoxy-7,8-diprenyl- flavone, 7,2′-dihydroxy-6,8-dimethyl-4′,5′-methylenedioxyflavan, and 5,7,4′-trihydroxy-3′-methoxy-6,8-di-Cmethylflavanone, were identified by ultra-performance liquid chromatography–tandem mass spectrometry. Our results suggest that the flavonoids of K. virginica root tubers might be potent antioxidants and can be effectively applied as an ingredient in food and natural medicine.


Kosteletzkya virginica; total flavonoid; antioxidant activity; UPLC-MS/MS

Cite This Article

Zai, Y., Liu, S. (2023). Extraction, Antioxidant Activity and Identification of Flavonoids from Root Tubers of Kosteletzkya virginica. Phyton-International Journal of Experimental Botany, 92(1), 225–236.

This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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