Open Access

ARTICLE

Screening of Bacillus subtilis HAAS01 and Its Biocontrol Effect on Fusarium wilt in Sweet Potato

by Chengyang Li^1,2,#, Lianjun Wang^1,#, Shasha Chai^1,#, Yinghua Xu^1,2, Chong Wang^1,2, Yi Liu^1,2, Jian Lei¹, Xiaojie Jin¹, Xianliang Cheng¹, Yuanyuan Yang¹, Xinsun Yang^1,*, Wenying Zhang^2,*

1 Food Crops Institute of Hubei Academy of Agricultural Sciences, Hubei Engineering and Technology Research Center of Sweet Potato, Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Wuhan, 430064, China
2 Research Center of Crop Stresses Resistance Technologies, Yangtze University, Jingzhou, 434025, China

* Corresponding Authors: Xinsun Yang. Email: ; Wenying Zhang. Email:
# These authors contributed equally to this work

Phyton-International Journal of Experimental Botany 2022, 91(8), 1779-1793. https://doi.org/10.32604/phyton.2022.020192

Received 10 November 2021; Accepted 06 January 2022; Issue published 14 April 2022

Abstract

Fusarium wilt, a disease caused by Fusarium oxysporum f.sp batatas (Fob) is an important disease in sweet potato production. Using endophytic bacteria for biological control of sweet potato diseases is one of the important ways. A Bacillus subtilis with antagonistic effect on Fusarium wilt of sweet potato was isolated from soil by confrontation culture. According to the biological characteristics, 16S rDNA sequence analysis, and physiological and biochemical analysis, the Bacillus subtilis HAAS01 was named. A pot experiment was conducted for the biological control experiment of strain HAAS01, and the endogenous hormone content, antioxidant enzyme activity, soluble protein content, and related gene expressions of sweet potato plants were detected. The results showed that the HAAS01 strain could promote the production of endogenous hormones and resist the infection of plant diseases together with defensive enzymes and upregulation of related gene expressions. In summary, Bacillus subtilis HAAS01 was effective in controlling Fusarium wilt of sweet potato and has potential for application and development.

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Keywords

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