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Quality control of ginseng mainly focuses on the contained saponins. In this work, the volatile components simultaneously from 12 Panax-derived herbal medicines were comparatively analyzed by an untargeted metabolomics approach using headspace solid-phase microextraction gas chromatography-mass spectrometry. Differential volatile components showed the potential to discriminate different ginseng species, which provided a new viewpoint for the precise quality control of ginseng.
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  • Open AccessOpen Access

    REVIEW

    Sorghum: Nutritional Factors, Bioactive Compounds, Pharmaceutical and Application in Food Systems: A Review

    Heba I. Mohamed1,*, Eman M. Fawzi1, Abdul Basit2, Kaleemullah3, Rafiq Lone4, Mahmoud R. Sofy5
    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1303-1325, 2022, DOI:10.32604/phyton.2022.020642
    Abstract After wheat, rice, maize, and barley, sorghum is the fifth most widely grown cereal on the planet. Due to its high production, drought resistance, and heat tolerance, this crop is replacing maize in some areas. Sorghum is available in a variety of colors, including cream, lemon-yellow, red, and even black. The principal grain anatomical components are pericarp, germ or embryo and endosperm. This review provides an overview of key sorghum grain components, including starches, fiber, proteins, lipids, and vitamins. Also, we summarized phenolic compounds, flavonoids, tannins, carotenoids, vitamin E, amines, Policosanols and Phytosterols in sorghum grains. Sorghum is used to… More >

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    REVIEW

    Formation Mechanism and Occurrence Law of Pod Shattering in Soybean: A Review

    Jinfeng Liu1, Yuzhe Zhang1, Yushi Jiang1, Hongji Sun1, Ruijie Duan1, Jing Qu2, Dan Yao1, Siyan Liu2,*, Shuyan Guan2,*
    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1327-1340, 2022, DOI:10.32604/phyton.2022.019870
    (This article belongs to this Special Issue: Integrating Agronomy and Plant Physiology for Improving Crop Production)
    Abstract Seed shattering refers to the phenomenon in which the pods split along the abdominal and back sutures before the crop is received, so that the seeds are spread. Seed shattering is vital to the reproduction of their offspring in wild plants, but it is also the main cause of crop yield loss reason. Pod-explosion resistance is a complex process of physical and physiological and biochemical reactions. Soybean seed shattering phenomenon is widespread, which severely restricts the development of soybean industry. Seed shattering (pod cracking or fruit dropping) is essential for the reproduction of its offspring in wild plants, but it… More >

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    ARTICLE

    Effects of Chemical Products on Fire Blight Suppression, and Fruit Production and Quality in Apple (Malus domestica Borkh.) cv. Golden Glory

    Álvaro Rodríguez-Peña1, Ana C. Gonzalez-Franco1, Jared Hernández-Huerta1, Nora A. Salas-Salazar1, Dámaris L. Ojeda-Barrios1, Esteban Sánchez2, Loreto Robles-Hernández1,*
    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1341-1351, 2022, DOI:10.32604/phyton.2022.019728
    Abstract Antibiotics are widely used in fire blight management programs, yet there are no studies that demonstrate the evaluation of their efficacy in Mexico. Therefore, the present study was conducted to investigate the effects of the active ingredients in five commercial products (Kasumin® 2L, Agrygent Plus®, Agricultural Terramycin®, Agrimicin® 100, and Actigard®) on fire blight suppression, and fruit yield and quality of apple (Malus domestica Borkh.) cv. Golden Glory. The experiment was conducted in a commercial orchard using a completely randomized block design, with six treatments: (1) Oxytetracycline [Ox], 110 mg L−1; (2) Kasugamycin [Kas], 4.7 mL L−1; (3) Oxytetracycline + Gentamicin [Ox… More >

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    ARTICLE

    Headspace Solid-Phase Micro-Extraction Gas Chromatography/Mass Spectrometry (HS-SPME-GC/MS)-Based Untargeted Metabolomics Analysis for Comparing the Volatile Components from 12 Panax Herbal Medicines

    Simiao Wang1,#, Xiaohang Li1,#, Meiting Jiang1, Xinlong Wu2, Yuying Zhao1, Meiyu Liu1, Xiaoyan Xu1, Huimin Wang1, Hongda Wang1, Heshui Yu2, Wenzhi Yang1,*
    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1353-1364, 2022, DOI:10.32604/phyton.2022.021093
    Abstract Quality control of ginseng currently is mainly based on ginsenoside analysis, but rarely focuses on the volatile organic components. In the current work, an untargeted metabolomics approach, by headspace solid-phase micro-extraction gas chromatography/mass spectrometry (HS-SPME-GC/MS), was elaborated and further employed to holistically compare the compositional difference of the volatile components simultaneously from 12 Panax herbal medicines, which included P. ginseng (PG), P. quinquefolius (PQ), P. notoginseng (PN), red ginseng (PGR), P. ginseng leaf (PGL), P. quinquefolius leaf (PQL), P. notoginseng leaf (PNL), P. ginseng flower (PGF), P. quinquefolius flower (PQF), P. notoginseng flower (PNF), P. japonicus (PJ), and P. japonicusMore >

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    ARTICLE

    Microalgae Improve the Photosynthetic Performance of Rice Seedlings (Oryza sativa L.) under Physiological Conditions and Cadmium Stress

    Ekaterina Yotsova1, Martin Stefanov1, Georgi Rashkov1, Margarita Kouzmanova2, Anelia Dobrikova1, Emilia Apostolova1,*
    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1365-1380, 2022, DOI:10.32604/phyton.2022.020566
    Abstract The aim of this study was to assess the impact of the microalgae Chlorella vulgaris on the rice seedlings at physiological conditions and under cadmium (Cd) stress. We examined the effects of C. vulgaris in the nutrient solution on rice seedlings grown hydroponically in the presence and the absence of 150 μM CdCl2, using the low (77 K) temperature and pulse amplitude modulated (PAM) chlorophyll fluorescence, P700 photooxidation measurements, photochemical activities of both photosystems, kinetic parameters of oxygen evolution, oxidative stress markers (MDA, H2O2 and proline), pigment content, growth parameters and Cd accumulation. Data revealed that the application C. vulgaris not… More >

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    ARTICLE

    Proteomics Analysis of Soybean Seedlings under Short-Term Water Deficit

    Xiyue Wang, Zihao Wu, Chao Yan, Chunmei Ma, Shoukun Dong*
    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1381-1401, 2022, DOI:10.32604/phyton.2022.020251
    Abstract Soybeans are one of the most important grain crops worldwide. Water deficit, which seriously affects the yield and quality of soybeans, is the main abiotic stress factor in soybean production. As a follow-up study, the droughttolerant soybean variant Heinong 44 was analyzed via proteome analysis. Soybean was exposed to water deficit for 0, 8, and 24 h, and protein samples were extracted for detection of differentially expressed proteins. Protein sequencing of leaf tissues under water stress yielded a total of 549 differentially expressed proteins: 75 and 320 upregulated proteins as well as 70 and 84 downregulated proteins were obtained after… More >

  • Open AccessOpen Access

    ARTICLE

    Proteomic Profiling and Protein-Protein Interaction Network Reveal the Molecular Mechanisms of Susceptibility to Drought Stress in Canola (Brassica napus L.)

    Reza Shokri-Gharelo1, Ali Bandehagh1,*, Mohammad Anwar Hossain2,*
    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1403-1417, 2022, DOI:10.32604/phyton.2022.020431
    Abstract Drought stress is one of the most important abiotic stresses that plants face frequently in nature. Under drought conditions, many morphological, physiological, and molecular aspects of plants are changed and as a result plants experience a remarkable reduction in growth, yield, and reproduction. To expand our understanding of the molecular basis of the plant response to drought stress, the proteomic profile and protein-protein network of canola (Brassica napus L.) were studied. The focus was to show molecular mechanisms related to canola susceptibility to drought stress. The experiment used a completely randomized design, implemented in a hydroponic system under greenhouse conditions.… More >

  • Open AccessOpen Access

    ARTICLE

    Phytohormones Accumulation and Distribution in Shoots and Roots of Haploid, Diploid and Tetraploid Barley Seedlings Derived from Microspore Culture

    Longhua Zhou1,2,#, Ting He1,2,#, Jing Li1,2,3, Guimei Guo1,2, Yingbo Li1,2, Hongwei Xu1,2, Runhong Gao1,2, Linli Huang1,2, Yifei Wang1,2, Ruiju Lu1,2, Zhiwei Chen1,2,*, Chenghong Liu1,2,*
    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1419-1428, 2022, DOI:10.32604/phyton.2022.019912
    Abstract Phytohormones play important roles in plant growth and development, and polyploids are thought to be an important method for plant breeding. However, the relationship between ploidy and phytohormone is still unclear. In this study, barley at three ploidy levels were produced by microspore culture. Therefore, we further analyzed the phytohormone content in the shoots and roots of the three kinds of barley materials to study the effect of ploidy on phytohormones accumulation and distribution. The results showed that Abscisic acid (ABA), gibberellin (GA), jasmonic acid (JA), auxin (IAA), salicylic acid (SA) and cytokinin (CTK) were successfully determined in shoots and… More >

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    ARTICLE

    Discovering Candidate Chromosomal Regions Linked to Kernel Size-Related Traits via QTL Mapping and Bulked Sample Analysis in Maize

    Hameed Gul1, Mengya Qian1, Mohammad G. Arabzai1,2, Tianhui Huang1, Qiannan Ma1, Fangyu Xing1, Wan Cao1, Tingting Liu1, Hong Duan1, Qianlin Xiao1,*, Zhizhai Liu1,*
    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1429-1443, 2022, DOI:10.32604/phyton.2022.019842
    (This article belongs to this Special Issue: Integrating Agronomy and Plant Physiology for Improving Crop Production)
    Abstract Kernel size-related traits, including kernel length, kernel width, and kernel thickness, are critical components in determining yield and kernel quality in maize (Zea mays L.). Dissecting the phenotypic characteristics of these traits, and discovering the candidate chromosomal regions for these traits, are of potential importance for maize yield and quality improvement. In this study, a total of 139 F2:3 family lines derived from EHel and B73, a distinct line with extremely low ear height (EHel), was used for phenotyping and QTL mapping of three kernel size-related traits, including 10-kernel length (KL), 10-kernel width (KWid), and 10-kernel thickness (KT). The results… More >

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    ARTICLE

    Bioinformatics Analysis of Disease Resistance Gene PR1 and Its Genetic Transformation in Soybeans and Cultivation of Multi-resistant Materials

    Huimin Cui, Shuo Qu, Abraham Lamboro, Yaolei Jiao, Piwu Wang*
    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1445-1464, 2022, DOI:10.32604/phyton.2022.020010
    Abstract In agricultural production, a single insect-resistant and disease-resistant variety can no longer meet the demand. In this study, the expression vector pCAMBIA-3301-PR1 containing the disease-resistant gene PR1 was constructed by means of genetic engineering, and the PR1 gene was genetically transformed to contain the PR1 gene through the pollen tube method. In CryAb-8Like transgenic high-generation T7 receptor soybean, a new material that is resistant to insects and diseases is obtained. For T2 transformed plants, routine PCR detection, Southern Blot hybridization, fluorescence quantitative PCR detection, indoor and outdoor pest resistance identification and indoor disease resistance identification were performed. The results showed… More >

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