Open Access iconOpen Access

ARTICLE

crossmark

Transcriptome Profiling of Barley Cultivar Hua 30 MDEC in Response to Agrobacterium Infection

Yingbo Li1,2,#, Ting He1,2,#, Guimei Guo1,2, Hongwei Xu1,2, Yingjie Zong1,2, Shuwei Zhang1,2, Ruiju Lu1,2, Longhua Zhou1,2,*, Chenghong Liu1,2,*

1 Biotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China
2 Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai, 201106, China

* Corresponding Authors: Longhua Zhou. Email: email; Chenghong Liu. Email: email

Phyton-International Journal of Experimental Botany 2022, 91(6), 1153-1164. https://doi.org/10.32604/phyton.2022.019520

Abstract

Agrobacterium-mediated transformation has been widely used in plants. However, the mechanism in plant cells’ response to Agrobacterium infection was very complex. The mechanism of the determinants in host cell remains obscure, especially in barley, which is recalcitrant for Agrobacterium-mediated transformation. In the present study, microspore-derived embryogenic calli (MDEC) from barley elite cultivar were employed as unique subjects to characterize the mechanisms during the Agrobacterium infection process. Hua 30 MDEC can be successfully infected by Agrobacterium. RNA-sequencing at different infection points (0, 2, 6, 12, 24 hpi) was performed. The average expressional intensity of the whole genomics increased from 0 to 2 hpi, and then decreased subsequently. More upregulated than downregulated differentially expressed genes (DEGs) were counted at the same time. GO enrichment analysis showed that protein modification was significantly overrepresented in upregulated DEGs. Chromosome-related biological processes, gene expression and cellular metabolic processes were significantly overrepresented in downregulated DEGs. KEGG analysis showed that plant defense responses, phenylpropanoid biosynthesis and biosynthesis of amino acids were significantly enriched across the infection time course. Nine DEGs related to defense responses were identified. All DEGs were upregulated from 2 to 24 hpi. We speculate that these genes are possibly related to Agrobacterium infection. These findings will provide deep insights into the molecular events occurring during the process of Agrobacterium-mediated transformation.

Keywords


Supplementary Material

Supplementary Material File

Cite This Article

Li, Y., He, T., Guo, G., Xu, H., Zong, Y. et al. (2022). Transcriptome Profiling of Barley Cultivar Hua 30 MDEC in Response to Agrobacterium Infection. Phyton-International Journal of Experimental Botany, 91(6), 1153–1164.



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 1773

    View

  • 1342

    Download

  • 0

    Like

Share Link