Open Access
ARTICLE
Transcriptome Profiling of Barley Cultivar Hua 30 MDEC in Response to Agrobacterium Infection
Yingbo Li1,2,#, Ting He1,2,#, Guimei Guo1,2, Hongwei Xu1,2, Yingjie Zong1,2, Shuwei Zhang1,2, Ruiju Lu1,2, Longhua Zhou1,2,*, Chenghong Liu1,2,*
1 Biotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China
2 Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai, 201106, China
* Corresponding Authors: Longhua Zhou. Email: ; Chenghong Liu. Email:
Phyton-International Journal of Experimental Botany 2022, 91(6), 1153-1164. https://doi.org/10.32604/phyton.2022.019520
Received 27 September 2021; Accepted 16 November 2021; Issue published 14 February 2022
Abstract
Agrobacterium-mediated transformation has been widely used in plants. However, the mechanism in plant cells’ response to
Agrobacterium infection was very complex. The mechanism of the determinants in host cell remains obscure, especially in barley, which is recalcitrant for
Agrobacterium-mediated transformation. In the present study, microspore-derived embryogenic calli (MDEC) from barley elite cultivar were employed as unique subjects to characterize the mechanisms during the
Agrobacterium infection process. Hua 30 MDEC can be successfully infected by
Agrobacterium. RNA-sequencing at different infection points (0, 2, 6, 12, 24 hpi) was performed. The average expressional intensity of the whole genomics increased from 0 to 2 hpi, and then decreased subsequently. More upregulated than downregulated differentially expressed genes (DEGs) were counted at the same time. GO enrichment analysis showed that protein modification was significantly overrepresented in upregulated DEGs. Chromosome-related biological processes, gene expression and cellular metabolic processes were significantly overrepresented in downregulated DEGs. KEGG analysis showed that plant defense responses, phenylpropanoid biosynthesis and biosynthesis of amino acids were significantly enriched across the infection time course. Nine DEGs related to defense responses were identified. All DEGs were upregulated from 2 to 24 hpi. We speculate that these genes are possibly related to
Agrobacterium infection. These findings will provide deep insights into the molecular events occurring during the process of
Agrobacterium-mediated transformation.
Keywords
Supplementary Material
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Cite This Article
Li, Y., He, T., Guo, G., Xu, H., Zong, Y. et al. (2022). Transcriptome Profiling of Barley Cultivar Hua 30 MDEC in Response to
Agrobacterium Infection.
Phyton-International Journal of Experimental Botany, 91(6), 1153–1164.