Open Access

ARTICLE

Cloning and Characterization of EuGID1 in Eucommia ulmoides Oliver

Yulu Chen¹, Fen Zhang¹, Ju Cai¹, Yichen Zhao^1,2, Jiaxue Cui¹, Yan Li^1,*
1 The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Institute of Agro-Bioengineering and College of Life Sciences, Guizhou University, Guiyang, 550025, China
2 College of Tea Sciences, Guizhou University, Guiyang, 550025, China
* Corresponding Author: Yan Li. Email:
(This article belongs to this Special Issue: Plant Omics in Challenging Environment)

Phyton-International Journal of Experimental Botany 2022, 91(5), 999-1013. https://doi.org/10.32604/phyton.2022.018984

Received 27 August 2021; Accepted 15 October 2021; Issue published 24 January 2022

Abstract

Gibberellic acid controlled the key developmental processes of the life cycle of landing plants, and regulated the growth and development of plants. In this study, a novel gibberellin receptor gene EuGID1 was obtained from Eucommia ulmoides Oliver. The cDNA of EuGID1 was 1556 bp, and the open reading frame was 1029 bp, which encoded 343 amino acids. EuGID1 had the homology sequence with the hormone-sensitive lipase family. Amino acid sequence alignment confirmed EuGID1 protein had the highest homology with the GID1 protein of Manihot esculenta. EuGID1 was located in the nucleus and cell membrane and had expression in four plant organs. Overexpression of EuGID1 in transgenic Arabidopsis plants promoted plant elongation and increased siliques yield.

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Keywords

Eucommia ulmoides Oliver; EuGID1 gene; overexpression; subcellular localization; heterologous transformation

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