TY - EJOU
AU - Yin, Wuzhong
AU - Yang, Hongxia
AU - He, Yantong Wang, Ping Feng, Yao Deng, Yang Liu, Danyang Chen, Yijie Ban, Weichi Liu, Guanghua
AU - Wang, Nan
TI - OsMAPK6 Affects Male Fertility by Reducing Microspore Number and Delaying Tapetum Degradation in Oryza sativa L.
T2 - Phyton-International Journal of Experimental Botany
PY - 2022
VL - 91
IS - 4
SN - 1851-5657
AB - The mitogen-activated protein kinase (MAPK) cascade is important in stress signal transduction and plant development. In the present study, we identified a rice (Oryza sativa L.) mutant with reduced fertility, Oryza sativa
mitogen-activated protein kinase 6 (osmapk6), which harbored a mutated MAPK gene. Scanning and transmission
electron microscopy, quantitative RT-PCR analysis, TUNEL assays, RNA in situ hybridization, longitudinal and
transverse histological sectioning, and map-based cloning were performed to characterize the osmapk6 mutant.
The gene OsMAPK6 was expressed throughout the plant but predominantly in the microspore mother cells, tapetal cells, and microspores in the anther sac. Compared with the wild type, the total number of microspores was
reduced in the osmapk6 mutant. The formation of microspore mother cells was reduced in the osmapk6 anther
sac at an early stage of anther development, which was the primary reason for the decrease in the total number of
microspores. Programmed cell death of some tapetal cells was delayed in osmapk6 anthers and affected exine formation in neighboring microspores. These results suggest that OsMAPK6 plays pivotal roles in microspore
mother cell formation and tapetal cell degradation.
KW - Mitogen-activated protein kinase; tapetal programmed cell death; microspore mother cell formation; male sterility; rice
DO - 10.32604/phyton.2022.017811