Overexpression of a Glycosyltransferase Gene from a Metabolically Poly-Resistant <i>Beckmannia syzigachne</i> Population Alters Growth and Confers Herbicide Resistance to <i>Brachypodium distachyon</i>

Yizhao Huang; Nannan Li; Deya Wang; Jian Du; Weitang Liu; Jinxin Wang; Wei Li

doi:10.32604/phyton.2022.018462

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Overexpression of a Glycosyltransferase Gene from a Metabolically Poly-Resistant Beckmannia syzigachne Population Alters Growth and Confers Herbicide Resistance to Brachypodium distachyon

Yizhao Huang¹, Nannan Li², Deya Wang⁴, Jian Du¹, Weitang Liu³, Jinxin Wang^3,*, Wei Li^1,*
1 College of Food Science and Pharmaceutical Engineering, Zaozhuang University, Zaozhuang, 277160, China
2 Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, 100093, China
3 Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Tai’an, 271018, China
4 College of Life Sciences, Zaozhuang University, Zaozhuang, 277160, China
* Corresponding Authors: Jinxin Wang. Email: ; Wei Li. Email:
(This article belongs to this Special Issue: Plant Ecophysiology: Recent Trends and Advancements)

Phyton-International Journal of Experimental Botany 2022, 91(4), 761-772. https://doi.org/10.32604/phyton.2022.018462

Received 26 July 2021; Accepted 26 September 2021; Issue published 09 December 2021

Abstract

Beckmannia syzigachne is a noxious weed for rice-wheat rotations in China. The B. syzigachne (AH-02) population evolved metabolic resistance to fenoxaprop-P-ethyl and mesosulfuron-methyl. To investigate the function of GT73C1 in this population, the GT73C1 gene was amplified by reverse transcription-polymerase chain reaction, and the sequence was 100% consistent with the transcriptome data. Its phylogenetic tree was displayed and annotated using FigTree v1.4.4. The plant overexpression vector of GT73C1 gene was constructed and used to transform Brachypodium distachyon plants. Furthermore, the expression of GT73C1 was significantly induced by fenoxaprop-P-ethyl and mesosulfuron-methyl, which was consistent with the findings from the whole plant bioassay. These results indicate that GT73C1 is closely related to the metabolic resistance of B. distachyon.

Keywords

Beckmannia syzigachne; GT73C1 gene; phylogenetic analyses; Brachypodium distachyon; genetic transformation

Cite This Article

Huang, Y., Li, N., Wang, D., Du, J., Liu, W. et al. (2022). Overexpression of a Glycosyltransferase Gene from a Metabolically Poly-Resistant Beckmannia syzigachne Population Alters Growth and Confers Herbicide Resistance to Brachypodium distachyon. Phyton-International Journal of Experimental Botany, 91(4), 761–772.

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