Open Access

ARTICLE

Effect of Putrescine on Low-Temperature Acclimation in Chlamydomonas reinhardtii

Muslum S. Inal^1,2, Dilek Unal^1,*, Bengu Turkyilmaz Unal^3,*, Munir Ozturk⁴

1 Molecular Biology and Genetics Department, Faculty of Science and Letter, Bilecik Şeyh Edebali University, Bilecik, 11230, Turkey
2 Biotechnology Application and Research Center, Bilecik Şeyh Edebali University, Bilecik, 11230, Turkey
3 Biotechnology Department, Art and Sciences Faculty, Niğde Ömer Halisdemir University, Niğde, 51240, Turkey
4 Botany Department, Centre for Environmental Studies, Ege University, Izmir, 35100, Turkey

* Corresponding Authors: Dilek Unal. Email: ; Bengu Turkyilmaz Unal. Email:

Phyton-International Journal of Experimental Botany 2022, 91(3), 583-598. https://doi.org/10.32604/phyton.2022.018223

Received 08 July 2021; Accepted 18 September 2021; Issue published 26 October 2021

Abstract

Putrescine is reported to be necessary for cold acclimation under low-temperature stress. In this study, the effect of low-temperature on some physiological and biochemical parameters has been investigated using the green algae Chlamydomonas reinhardtii. The lipid peroxidation rate, amount of Rubisco protein, activities of antioxidant enzymes and gene expression of polyamine biosynthesis (odc2, and spd1), heat shock proteins (hsp70c, hsp90a, and hsp90c), and PSII repair mechanisms (psba, rep27, and tba1) were determined to understand the low-temperature response. Exogenous putrescine application significantly increased Rubisco protein concentration and catalase enzyme activities under low-temperature stress. Moreover, real-time RT-PCR results and gene expression analysis showed that polyamine metabolism induced gene expression at low-temperatures in the first 24 h. In the same way, the gene expression of heat shock proteins (hsp70c, hsp90a, and hsp90c) decreased under low-temperature treatment for 72 h; however, application of putrescine enhanced the gene expression in the first 24 h. The results obtained indicated that molecular response in the first 24 h could be important for cold acclimation. The psba and tba1 expressions were reduced under low-temperatures depending on the exposure time. In contrast, the exogenous putrescine enhanced the expression level of the psba response to low-temperature at 24 and 72 h. The results obtained in this study indicate that putrescine could play a role in the PS II repair mechanisms under low-temperature stress.

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