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Diversity Assessment of an Endemic Carpinus oblongifolia (Betulaceae) Using Specific-Locus Amplified Fragment Sequencing and Implications for Conservation

Sumei Li, Qing Wang, Peng Wang, Shuan Wang, Rutong Yang, Ya Li*
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing Botanical Garden Memorial Sun Yat-Sen, Nanjing, 210014, China
* Corresponding Author: Ya Li. Email:
(This article belongs to this Special Issue: Plant Ecophysiology: Recent Trends and Advancements)

Phyton-International Journal of Experimental Botany 2022, 91(3), 617-632. https://doi.org/10.32604/phyton.2022.017741

Received 02 June 2021; Accepted 17 August 2021; Issue published 26 October 2021

Abstract

Carpinus oblongifolia is an endemic species and the extant wild populations show a fragmentation distribution in the Baohua Mountain of Jiangsu Province in eastern China. Understanding of genetic diversity plays an important role in C. oblongifolia survival and sustainable development. The wild C. oblongifolia population was artificially divided into four subpopulations according to the microhabitats, and another two subpopulations were constructed by progeny seedlings cultivated with the mature seeds. Then, the leaf buds of 80 individuals from six subpopulations were sampled to develop single nucleotide polymorphisms (SNPs) using specific-locus amplified fragment sequencing (SLAF-seq). Based on these SNPs, we aimed to characterize the genetic diversity and population structure of C. oblongifolia and provide an illumination and reference for effective management of such a small endemic population. The level of genetic diversity was low at the species level, and the progeny subpopulations had a relatively higher genetic diversity than the wild subpopulations. This may be attributed to a high gene flow and an excess heterozygosity to reduce the threat of genetic drift-based hazards. Moreover, the progeny subpopulations had the ability to form new clusters and a great contribution to the genetic structure variation of C. oblongifolia. These results will assist with the development of conservation and management strategies, such as properly evacuating competitive trees to provide more chance for pollen and seed flow in situ conservation, and establishing sufficient seedling plantlets under laboratory conditions for reintroduction to enlarge the effective population size.

Keywords

Carpinus oblongifolia; endemic species; genetic diversity; population structure; SLAF

Cite This Article

Li, S., Wang, Q., Wang, P., Wang, S., Yang, R. et al. (2022). Diversity Assessment of an Endemic Carpinus oblongifolia (Betulaceae) Using Specific-Locus Amplified Fragment Sequencing and Implications for Conservation. Phyton-International Journal of Experimental Botany, 91(3), 617–632.



This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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