Open Access

ARTICLE

Global Lysine Acetylome Analysis of Flower Bud Development in Catalpa bungei

Danlong Jing^1,2, Nan Wang¹, Nan Lu¹, Guanzheng Qu³, Guolu Liang², Wenjun Ma¹, Shougong Zhang^1,*, Junhui Wang^1,*

1 State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China
2 Key Laboratory of Horticulture Science for Southern Mountains Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China
3 State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150040, China

* Corresponding Authors: Shougong Zhang. Email: ; Junhui Wang. Email:

Phyton-International Journal of Experimental Botany 2022, 91(3), 507-524. https://doi.org/10.32604/phyton.2022.017644

Received 26 May 2021; Accepted 09 July 2021; Issue published 26 October 2021

Abstract

Lysine acetylation, a reversible and dynamic post-translational modification, plays pivotal roles in regulating many biological processes in plants. However, the information of lysine acetylation during flower development of woody angiosperm remains unclear. Herein, we identified a total of 667 lysine acetylation sites in 467 proteins in flower buds of Catalpa Bungei. The acetylated proteins were mainly involved in the pathways of carbon metabolism, glycometabolism and oxidative phosphorylation. Using functional enrichment analysis, 61 and 13 acetylated proteins were involved in the glycometabolism and oxidative phosphorylation pathways, respectively. This suggests that lysine acetylated proteins play critical roles in the energy metabolism pathways. Accordingly, the protein–protein interactions were also highly enriched in the carbon metabolic pathway. Integrative analysis of lysine acetylome and transcriptome showed that transcript level changes of seven key lysine-acetylated proteins/genes involved in energy metabolism were significant different during floral transition. Importantly, compared with the normal flowering variety, the expression levels of phosphoribulokinase, phosphoenolpyruvate carboxykinase, ATP synthase and pyruvate dehydrogenase complex genes were significantly up-regulated at the stages of vegetative and transition buds during early flowering of C. bungei. These data represent the first lysine acetylome in the C. bungei flowers, and can serve as an essential basis for exploring the function of lysine acetylated proteins in the flower bud development of plants.

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Keywords

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Supplementary Material

Supplementary Material File

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