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Proteomic Analysis of Chrysanthemum Lateral Buds after Removing Apical Dominance Based on Label-Free Technology

Sicong Zheng#, Jingjing Song#, Cheng Luo, Xin Li, Qiqi Ma, Beibei Jiang*, Qinglin Liu, Yuanzhi Pan
Department of Ornamental Horticulture, Sichuan Agricultural University, Chengdu, 611130, China
# These authors contributed equally to this work
* Corresponding Author: Beibei Jiang. Email:
(This article belongs to this Special Issue: Plant Omics in Challenging Environment)

Phyton-International Journal of Experimental Botany 2022, 91(3), 525-539. https://doi.org/10.32604/phyton.2022.017629

Received 25 May 2021; Accepted 13 August 2021; Issue published 26 October 2021

Abstract

Studying the genetic basis and regulatory mechanism of chrysanthemum lateral bud outgrowth is of great significance for reduction the production cost of cut chrysanthemum. To clarify the molecular basis of lateral bud elongation after removal of apical dominance in chrysanthemum, label-free quantification analysis was used to analyze the proteome changes after apical bud removal. Quantitative real-time PCR (qPCR) was used to analyze the changes in the expression of three plant hormone-related genes. A total of 440 differentially expressed proteins were successfully identified at three time points during the lateral bud elongation. The number of differentially expressed proteins in the three stages (24 h/0 h, 48 h/0 h, 48 h/24 h) were 219, 332, and 97, respectively. The difference in expressed proteins in the three comparison stages mainly involves RNA processing and modification; translation, ribosomal structure and biogenesis; Posttranslational modification, protein turnover, and chaperones. Path analysis showed that there was various physiological activities in the process of lateral bud dormancy breaking and elongation, which involved energy metabolism, biosynthesis, signal transduction and stress response in the growth process of lateral buds. qPCR indicated that the expression of cytokinin synthesis related gene was significantly increased after the removal of apical dominance, while the expression of strigolactones synthesis related gene experiences a dramatic fall to promote the development of the lateral buds. However, there was a drop before a slight increase in the expression of the auxin synthesis related gene, which was mainly due to the removal of apical dominance that led to the loss of indoleacetic acid in the main stem. However, with formation of the new apical source, indoleacetic acid can be released again.

Keywords

Branches; proteins; decapitation; proteome; 4D label-free quantification analysis; quantitative real-time PCR

Cite This Article

Zheng, S., Song, J., Luo, C., Li, X., Ma, Q. et al. (2022). Proteomic Analysis of Chrysanthemum Lateral Buds after Removing Apical Dominance Based on Label-Free Technology. Phyton-International Journal of Experimental Botany, 91(3), 525–539.



This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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