Open Access

ARTICLE

Nitric Oxide Regulates Mitochondrial Fatty Acids and Promotes CBF Expression of Peach Fruit during Cold Storage

by Xiaoshan Guo^1,2,#, Dandan Huang^2,#, Siyu Wen², Yang Bai², Shuhua Zhu^2,*, Jianrong Feng^1,*

1 Department of Horticulture, College of Agriculture, Shihezi University, Shihezi, 832000, China
2 College of Chemistry and Material Science, Shandong Agricultural University, Taian, 271018, China

* Corresponding Authors: Shuhua Zhu. Email: ; Jianrong Feng. Email:

Phyton-International Journal of Experimental Botany 2022, 91(2), 409-427. https://doi.org/10.32604/phyton.2022.018022

Received 23 June 2021; Accepted 09 July 2021; Issue published 26 September 2021

Abstract

Low-temperature storage is convenient for postharvest preservation of peach fruit, but peach fruit is sensitive to cold damage, which lowers its quality. Nitric oxide (NO) has the potential to improve the bitter resistance of peach fruit. In this work, peach fruit was treated with 15 μmol L⁻¹ NO and 5 μmol L⁻¹ c-PTIO [2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxo-3-oxide], to study changes in mitochondrial fatty acids and expression of the C-repeat binding factor (CBF). The results showed that 15 μmol L⁻¹ exogenous NO significantly maintained fruit quality, reduced peroxidation of mitochondrial fatty acids, increased the activities of the antioxidants superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbic acid peroxidase (APX), and reduced the content of hydrogen peroxide (H₂O₂). Meanwhile, NO treatment suppressed the increase in browning index and ion leakage rate, increased the activity of S-nitrosoglutathione reductase (GSNOR), the contents of S-nitrosothiols (SNOs), and the ratios of mitochondrial NAD⁺/NADH and NADP⁺/NADPH, increased the expression levels of PpCBF1/5/6. However, the expression levels of PpCBF2/3/4 were not significantly regulated by exogenous NO. Peaches treated with c-PTIO showed opposite effects to those treated with exogenous NO. These results suggest that exogenous NO can improve antioxidant capacity, preserve mitochondrial fatty acids, and upregulate the expression of PpCBF1/5/6 to alleviate cold tolerance and maintain the peach quality during storage.

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Keywords

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