Open Access

ARTICLE

Transcriptome Analysis via RNA Sequencing Reveals the Molecular Mechanisms Underlying the Hedera helix Response to High Temperature

by Ting Zhang^1,2, Ping Li^3,*, Jiali Wei^3,*

1 Shanghai Botanical Garden, Shanghai, 200231, China
2 Shanghai Engineering Research Center of Sustainable Plant Innovation, Shanghai, 200231, China
3 Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai, 200444, China

* Corresponding Authors: Ping Li. Email: ; Jiali Wei. Email:

(This article belongs to the Special Issue: Plant–Environment Interactions)

Phyton-International Journal of Experimental Botany 2022, 91(11), 2403-2417. https://doi.org/10.32604/phyton.2022.022421

Received 09 March 2022; Accepted 23 April 2022; Issue published 12 July 2022

Abstract

Hedera helix is an evergreen ornamental plant that is resistant to cool but not high temperature and deserves to be further researched for improving its adaptability to heat stress. Two Hedera helix cultivars, heat-tolerant (HT) ‘Jessica’ and heat-sensitive (HS) ‘Shamrock’, were used for differences analyses of transcriptome. We detected 6179 differentially expressed genes (DEGs) and 5992 DEGs in ‘Jessica’ and ‘Shamrock’ to heat stress, respectively. Among these, 1983 upregulated DEGs and 1400 downregulated DEGs were shared between both varieties, resulting in enhancement of various pathways such as biosynthesis of secondary metabolites, glyoxylate dicarboxylate metabolism, and protein processing in endoplasmic reticulum (PPER), RNA transport, respectively. Among the common downregulated DEGs, 72 TFs in 25 gene families were found, including members of the MYB and MYB-related families, the bHLH family, etc. In ‘Jessica’ (HT), 634 unique up-regulated DEGs were identified, including genes associated with phenylpropanoid biosynthesis, starch and sucrose metabolism, biosynthesis of amino acids. Most upregulated TFs of HT were upregulated much more rapidly than those of HS in response to high temperature. Eleven TF-encoding genes were selected to verify the RNA sequencing data by qPCR. This study revealed the gene expression patterns of ivy in response to heat stress and the molecular basis of heat tolerance, which provided theoretical references for improving the heat tolerance of ivy.

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Keywords

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Supplementary Material

Supplementary Material File

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