Open Access
ARTICLE
In vitro Germination and Micropropagation of Aconitum vilmorinianum: An Important Medicinal Plant in China
Zongmin Mou1,2,3, Fei Ye4, Fangchen Shen1, Dake Zhao1,2,3,*
1
School of Ecology and Environmental Science, Yunnan University, Kunming, 650504, China
2
Biocontrol Engineering Research Center of Plant Disease and Pest, Yunnan University, Kunming, 650504, China
3
Biocontrol Engineering Research Center of Crop Disease and Pest, Yunnan University, Kunming, 650504, China
4
Kunming Municipal Hospital of Traditional Chinese Medicine, Kunming, 650500, China
* Corresponding Author: Dake Zhao. Email:
(This article belongs to this Special Issue: Biotechnology of Plant Secondary Metabolites)
Phyton-International Journal of Experimental Botany 2022, 91(10), 2331-2348. https://doi.org/10.32604/phyton.2022.021273
Received 05 January 2022; Accepted 21 February 2022; Issue published 30 May 2022
Abstract
Aconitum vilmorinianum, a well-known traditional Chinese herb, is recently being threatened by overexploitation
and environment disturbance. This study was conducted to provide propagation methods through
in vitro germination and explant cultivation. Germination was stimulated up to 66.00% on Murashige and Skoog (MS) medium containing 2.0 mg L
−1 6-benzylaminopurine (BAP), 0.1 mg L
−1 1-napthaleneacetic acid (NAA), and 30 g L
−1
sucrose. Three bacteria (
Pantoea agglomerans,
Erwinia persicina, and
Pseudomonas tolaasii) would be responsible
for consistent contamination during germination. The latter two were effectively eradicated after disinfected. The
influence of explant types and hormone combinations on direct and indirect organogenesis was evaluated in the
present work. The frequency of shoot induction from axillary bud explants was 100% on the MS fortified with
2.0 mg L
−1 BAP and 0.3 mg L
−1 NAA. Shoots multiplication was optimized on MS medium supplemented with
0.1 mg L
−1 thidiazuron (TDZ) and 0.1 mg L
−1 NAA. High callus induction percentage (96.67%) was obtained
from stem segments on MS medium with 2.0 mg L
−1 2,4-D, then successfully regenerated into shoots on MS medium in the presence of 0.1 mg L
−1 TDZ and 0.2 mg L
−1 NAA. The present work could be useful for the utilization
and conservation of this valuable species.
Keywords
Cite This Article
Mou, Z., Ye, F., Shen, F., Zhao, D. (2022).
In vitro Germination and Micropropagation of
Aconitum vilmorinianum: An Important Medicinal Plant in China.
Phyton-International Journal of Experimental Botany, 91(10), 2331–2348.