Open Access

ARTICLE

Encapsulation of Immature Somatic Embryos of Coffea arabica L. for in Vitro Preservation

Eliana Arias-Pérez¹, Carlos Alberto Lecona-Guzmán¹, Federico Antonio Gutiérrez-Miceli¹, Joaquín Adolfo Montes-Molina¹, Nancy Ruiz-Lau^1,2,*
1 Laboratorio de Biotecnología Vegetal, Instituto Tecnológico de Tuxtla Gutiérrez, Tecnológico Nacional de México, Tuxtla Gutiérrez, Chiapas, 29050, México
2 CONACYT-Tecnológico Nacional de México/Instituto Tecnológico de Tuxtla Gutiérrez, Tuxtla Gutiérrez, Chiapas, 29050, México
* Corresponding Author: Nancy Ruiz-Lau. Email:
(This article belongs to this Special Issue: Tree Somatic Embryogenesis and Application)

Phyton-International Journal of Experimental Botany 2021, 90(6), 1741-1748. https://doi.org/10.32604/phyton.2021.016004

Received 30 January 2021; Accepted 29 April 2021; Issue published 28 June 2021

Abstract

The present study aimed to develop a protocol for somatic embryogenesis and encapsulation of coffee embryos (Coffea arabica L.), for the conservation of genotypes with characteristics of commercial interest. Somatic embryos were induced from leaf explants in Murashige and Skoog medium (MS) supplemented with 1 mg · L⁻¹ of 2,4-dichlorophenoxiacetic acid (2,4-D) combined with 2 mg · L⁻¹ of benzyladenine (BA). Somatic embryos (SE) at the globular stage were encapsulated in a sodium alginate matrix; two treatments were tested: MS + 5 mg · L⁻¹ BA + 1 mg · L⁻¹ NAA + 3% (w/v) alginate, and MS + 7 mg · L⁻¹ BA + 5.7 mg · L⁻¹ indoleacetic acid (IAA) + 3% (w/v) alginate. Alginate was complexed with 100 mM calcium chloride (CaCl₂). Viability of the encapsulated SE was determined by staining with 0.01% fluorescein diacetate (FDA) after 0, 15, 30, and 45 days of storage at 4°C. Embryo viability was 100% in both treatments.

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Keywords

Coffee; explants; embryo; artificial seeds

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