Open Access
ARTICLE
Encapsulation of Immature Somatic Embryos of Coffea arabica L. for in Vitro Preservation
Eliana Arias-Pérez1, Carlos Alberto Lecona-Guzmán1, Federico Antonio Gutiérrez-Miceli1, Joaquín Adolfo Montes-Molina1, Nancy Ruiz-Lau1,2,*
1 Laboratorio de Biotecnología Vegetal, Instituto Tecnológico de Tuxtla Gutiérrez, Tecnológico Nacional de México, Tuxtla Gutiérrez, Chiapas, 29050, México
2 CONACYT-Tecnológico Nacional de México/Instituto Tecnológico de Tuxtla Gutiérrez, Tuxtla Gutiérrez, Chiapas, 29050, México
* Corresponding Author: Nancy Ruiz-Lau. Email:
(This article belongs to the Special Issue: Tree Somatic Embryogenesis and Application)
Phyton-International Journal of Experimental Botany 2021, 90(6), 1741-1748. https://doi.org/10.32604/phyton.2021.016004
Received 30 January 2021; Accepted 29 April 2021; Issue published 28 June 2021
Abstract
The present study aimed to develop a protocol for somatic embryogenesis and encapsulation of coffee embryos (
Coffea arabica L.), for the conservation of genotypes with characteristics of commercial interest. Somatic embryos were induced from leaf explants in Murashige and Skoog medium (MS) supplemented with 1 mg · L
−1 of 2,4-dichlorophenoxiacetic acid (2,4-D) combined with 2 mg · L
−1 of benzyladenine (BA). Somatic embryos (SE) at the globular stage were encapsulated in a sodium alginate matrix; two treatments were tested: MS + 5 mg · L
−1 BA + 1 mg · L
−1 NAA + 3% (w/v) alginate, and MS + 7 mg · L
−1 BA + 5.7 mg · L
−1 indoleacetic acid (IAA) + 3% (w/v) alginate. Alginate was complexed with 100 mM calcium chloride (CaCl
2). Viability of the encapsulated SE was determined by staining with 0.01% fluorescein diacetate (FDA) after 0, 15, 30, and 45 days of storage at 4°C. Embryo viability was 100% in both treatments.
Keywords
Cite This Article
APA Style
Arias-Pérez, E., Lecona-Guzmán, C.A., Gutiérrez-Miceli, F.A., Montes-Molina, J.A., Ruiz-Lau, N. (2021). Encapsulation of immature somatic embryos of coffea arabica L. for in vitro preservation. Phyton-International Journal of Experimental Botany, 90(6), 1741-1748. https://doi.org/10.32604/phyton.2021.016004
Vancouver Style
Arias-Pérez E, Lecona-Guzmán CA, Gutiérrez-Miceli FA, Montes-Molina JA, Ruiz-Lau N. Encapsulation of immature somatic embryos of coffea arabica L. for in vitro preservation. Phyton-Int J Exp Bot. 2021;90(6):1741-1748 https://doi.org/10.32604/phyton.2021.016004
IEEE Style
E. Arias-Pérez, C.A. Lecona-Guzmán, F.A. Gutiérrez-Miceli, J.A. Montes-Molina, and N. Ruiz-Lau "Encapsulation of Immature Somatic Embryos of Coffea arabica L. for in Vitro Preservation," Phyton-Int. J. Exp. Bot., vol. 90, no. 6, pp. 1741-1748. 2021. https://doi.org/10.32604/phyton.2021.016004