@Article{phyton.2021.016143, AUTHOR = {Xin Li, Feiya Liao, Qiqi Ma, Beibei Jiang, Yuanzhi Pan, Cheng Luo, Xinjie Wang, Aining Ran}, TITLE = {Proteomic Analysis of High Temperature Stress-Responsive Proteins in Chrysanthemum Leaves}, JOURNAL = {Phyton-International Journal of Experimental Botany}, VOLUME = {90}, YEAR = {2021}, NUMBER = {5}, PAGES = {1415--1423}, URL = {http://www.techscience.com/phyton/v90n5/42619}, ISSN = {1851-5657}, ABSTRACT = {Chrysanthemum is one of the most important ornamental flowers in the world, and temperature has a significant influence on its field production. In the present study, differentially expressed proteins were investigated in the leaves of Dendranthema grandiflorum ‘Jinba’ under high temperature stress using label-free quantitative proteomics techniques. The expressed proteins were comparatively identified and analyzed. A total of 1,463 heat-related, differentially expressed proteins were successfully identified by Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS), and 1,463 heat-related, differentially expressed proteins were successfully identified by mass spectrometry after a high temperature treatment. Among these, 701 proteins were upregulated and 762 proteins were downregulated. The in-depth bioinformatics analysis of these differentially expressed proteins revealed that these were involved in energy metabolism pathways, protein metabolism, and heat shock. In the present study, the investigators determined the changes in the levels of some proteins, and their expression at the protein and molecular levels in chrysanthemum to help reveal the mechanism of heat resistance in chrysanthemum. Furthermore, the present study elucidated some of the proteins correlated to heat resistance in chrysanthemum, and their expression changes at the protein and molecular levels to help reveal the mechanism of heat resistance in this flower species. These results provide a theoretical basis for the selection of new heat resistant varieties of chrysanthemum in the field.}, DOI = {10.32604/phyton.2021.016143} }