Open Access

ARTICLE

Quantitative Proteomics Analysis Identifies the Potential Mechanism Underlying Yellow-Green Leave Mutant in Wheat

Wei Zheng, Zheng Shi, Mei Long, Yuncheng Liao^*
College of Agronomy, Northwest A&F University, Yangling, 712100, China
* Corresponding Author: Yuncheng Liao. Email:
(This article belongs to this Special Issue: Plant Physiology for Crop Production and Sustainable Agriculture)

Phyton-International Journal of Experimental Botany 2021, 90(4), 1147-1159. https://doi.org/10.32604/phyton.2021.015916

Received 23 January 2021; Accepted 19 March 2021; Issue published 27 April 2021

Abstract

Enhancing photosynthesis efficiency is considered as one of the most crucial targets during wheat breeding. However, the molecular basis underlying high photosynthesis efficiency is not well understood up to now. In this study, we investigated the protein expression profile of wheat Jimai5265yg mutant, which is a yellow-green mutant with chlorophylls b deficiency but high photosynthesis efficiency. Though TMT-labeling quantitative proteomics analysis, a total of 72 differential expressed proteins (DEPs) were obtained between the mutant and wild type (WT). GO analysis found that they significantly enriched in thylakoid membrane, pigment binding, magnesium chelatase activity and response to light intensity. KEGG analysis showed that they involved in photosynthesis-antenna protein as well as porphyrin and chlorophyll metabolism. Finally, 118 RNA editing events were found between mutant and WT genotype. The A to C editing in the 3-UTR of TraesCS6D02G401500 lead to its high expression in mutant through removing the inhibition of tae-miR9781, which might have vital role in regulating the yellow-green mutant. This study provided some useful clues about the molecular basis of Jimai5265yg mutant as well as chlorophylls metabolism in wheat.

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Keywords

Wheat; photosynthesis; yellow-green mutant; quantitative proteomics

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