Open Access

ARTICLE

Cloning and Bioinformatics Analysis of the GlROP6 gene in Glehnia littoralis

Min Yan¹, Han Wang¹, Shaohua Liu¹, Ye Xu¹, Zizhuo Nie¹, Yifeng Zhou², Li Li^2,*
1 Jiangsu Key Laboratory of Biofunctional Molecules, School of Life Sciences, Chemistry & Chemical Engineering, Jiangsu Second Normal University, Nanjing, 211200, China
2 Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing, 210014, China.
* Corresponding Author: Li Li. Email:
(This article belongs to this Special Issue: Plant Ecophysiology: Recent Trends and Advancements)

Phyton-International Journal of Experimental Botany 2021, 90(4), 1293-1300. https://doi.org/10.32604/phyton.2021.015601

Received 30 December 2020; Accepted 24 February 2021; Issue published 27 April 2021

Abstract

Rho-related GTPase from plants (ROP) proteins play an essential role in plant stress resistance. In this study, the full-length GlROP6 gene was cloned based on G. littoralis transcriptome sequencing data acquired in response to salt stress. The protein sequence, conserved domains, secondary structure, three-dimensional structure, phylogenetic relationships, and expression pattern of the GlROP6 gene were systematically analysed. Our results showed that the full-length GlROP6 gene had an open reading frame of 606 bp, which encoded 201 amino acid residues with a relative molecular weight of 22.23463 kDa and a theoretical isoelectric point of 9.06. Amino acid sequence analyses indicated that the structure of the GlROP6 protein was conserved, and included five G-box motifs (G1–G5), an effector binding region, a Rho insert region and a C-terminal hypervariable region. According to our phylogenetic analysis, the GlROP6 protein was closely related to the ROP protein of Daucus carota subsp. Sativus. Our quantitative real-time PCR results revealed that GlROP6 was highly expressed in flower, and GlROP6 expression was significantly upregulated in G. littoralis roots treated with NaCl. This study will facilitate investigations into the function of GlROP genes in response to salt stress in G. littoralis.

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Keywords

GlROP6 gene; Glehnia littoralis; salt stress; expression analysis

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