Open Access

ARTICLE

Non-Polar Chemical Constituents of Atemoya and Evaluation of the Cytotoxic and Antimicrobial Activity

Suzana Vieira Rabêlo¹, Fernanda Granja da Silva Oliveira², Michele Miriam Calixto de Lira³, Livia Macedo Dutra³, Adilson Sartoratto⁴, Marta Cristina Teixeira Duarte⁴, Maria Claudia dos Santos Luciano⁵, Maria Francilene Souza Silva⁵, Cláudia do Ó Pessoa⁵, Manoel Odorico de Moraes Filho⁵, Emmanoel Vilaça Costa⁶, Jackson Roberto Guedes da Silva Almeida^1,3,*

1 Post-Graduate Program in Biotechnology (RENORBIO), Federal Rural University of Pernambuco, Recife, Pernambuco, 52171-900, Brazil
2 Post-Graduate Program in Biotechnology, State University of Feira de Santana, Feira de Santana, Bahia, 44036-900, Brazil
3 Center for Studies and Research of Medicinal Plants (NEPLAME), Federal University of San Francisco Valley, Petrolina, Pernambuco, 56304-205, Brazil
4 Multidisciplinary Center of Chemical, Biological and Agricultural Research (CPQBA), Campinas State University, Campinas, São Paulo, 13083-970, Brazil
5 National Laboratory of Experimental Oncology (LabNOE), Federal University of Ceará, Fortaleza, Ceará, 60020-181, Brazil
6 Departament of Chemistry, Federal University of Amazonas, Manaus, Amazonas, 69067-005, Brazil

* Corresponding Author: Jackson Roberto Guedes da Silva Almeida. Email:

Phyton-International Journal of Experimental Botany 2021, 90(3), 921-931. https://doi.org/10.32604/phyton.2021.013305

Received 01 August 2020; Accepted 01 December 2020; Issue published 30 March 2021

Abstract

This study aimed to identify the chemical composition of essential oil from fruits (EOAF) and the hexanic crude extract from aerial parts (At-Hex) of atemoya (Annona cherimola x Annona squamosa), a hybrid belonging to the Annonaceae family. Cytotoxic and antimicrobial activity was also evaluated. OEAF was obtained by hydrodistillation using a Clevenger apparatus, and their composition was analyzed by gas chromatography-mass spectrometry (GC-MS) analyses. Cytotoxicity was tested against human tumor cell lines HCT-116 (colon carcinoma), SF-295 (glioblastoma), OVCAR-8 (ovarian carcinoma) and HL60 (leukemia) using 3-(4,5-dimethyl-2-thiazolyl)-2,5- diphenyl-2H-tetrazolium bromide (MTT) assay, while antimicrobial activity was conducted by bioauthography method against eleven microorganisms strains. Twenty-four compounds were identified in the EOAF and twenty-nine in At-Hex. The monoterpenes linalool (25.70%), α-pinene (10.38%), β-pinene (9.12%), transocimene (7.43%), and the sesquiterpene bicyclogermacrene (12.58%) were the major constituents of EOAF, whereas the sesquiterpene spathulenol (13.91%) was the main compound of At-Hex. At-Hex showed a high cytotoxicity against SF-295 (glioblastoma). These findings show an important chemotaxonomic contribution for the Annonaceae family, mainly for the Annona genus. Atemoya proved to be a promising source of substances with potential cytotoxic activity.

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