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ARTICLE

Differential Responses of NHX1 and SOS1 Gene Expressions to Salinity in two Miscanthus sinensis Anderss. Accessions with Different Salt Tolerance

Qian Sun^1,2, Toshihiko Yamada³, Yulai Han^2,*, Tetsuo Takano^1,*

1 Asian Natural Environmental Science Center (ANESC), The University of Tokyo, Tokyo, 188-0002, Japan
2 College of Health Science and Environmental Engineering, Shenzhen Technology University, Shenzhen, 518118, China
3 Field Science Center for Northern Biosphere, Hokkaido University, Sapporo, 060-0811, Japan

* Corresponding Authors: Yulai Han. Email: ; Tetsuo Takano. Email: -tokyo.ac.jp

Phyton-International Journal of Experimental Botany 2021, 90(3), 827-836. https://doi.org/10.32604/phyton.2021.013805

Received 20 August 2020; Accepted 01 December 2020; Issue published 30 March 2021

Abstract

The lignocellulosic crop Miscanthus spp. has been identified as a good candidate for biomass production. The responses of Miscanthus sinensis Anderss. to salinity were studied to satisfy the needs for high yields in marginal areas and to avoid competition with food production. The results indicated that the relative advantages of the tolerant accession over the sensitive one under saline conditions were associated with restricted Na⁺ accumulation in shoots. Seedlings of two accessions (salt-tolerant ‘JM0119’ and salt-sensitive ‘JM0099’) were subjected to 0 (control), 100, 200, and 300 mM NaCl stress to better understand the salt-induced biochemical responses of genes involved in Na⁺ accumulation in M. sinensis. The adaptation responses of genes encoding for Na⁺ /H⁺ antiporters, NHX1 and SOS1 to NaCl stress were examined in JM0119 and JM0099.The cDNA sequences of genes examined were highly conserved among the relatives of M. sinensis based on the sequencing on approximate 600 bp-long cDNA fragments obtained from degenerate PCR. These salt-induced variations of gene expression investigated by quantitative real-time PCR provided evidences for insights of the molecular mechanisms of salt tolerance in M. sinensis. The expression of NHX1 was up-regulated by salt stress in JM0119 shoot and root tissues. However, it was hardly affected in JM0099 shoot tissue except for a significant increase at the 100 mM salt treatment, and it was salt-suppressed in the JM0099 root tissue. In the root tissue, the expression of SOS1 was induced by the high salt treatment in JM0119 but repressed by all salt treatments in JM0099. Thus, the remarkably higher expression of NHX1 and SOS1 were associated with the resistance to Na⁺ toxicity by regulation of the Na⁺ influx, efflux, and sequestration under different salt conditions.

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