Open Access

ARTICLE

In vitro Antibacterial Activity of Moringa oleifera Ethanolic Extract against Tomato Phytopathogenic Bacteria

Roberto Arredondo-Valdés¹, Francisco D. Hernández-Castillo², Mario Rocandio-Rodríguez¹, Julia C. Anguiano-Cabello³, Madai Rosas-Mejía¹, Venancio Vanoye-Eligio¹, Salvador Ordaz-Silva⁴, Imelda V. López-Sánchez⁴, Laura D. Carrazco-Peña⁴, Julio C. Chacón-Hernández^1,*
1 Institute of Applied Ecology, Universidad Autónoma de Tamaulipas, Ciudad Victoria, Tamaulipas, 87019, México
2 Parasitology Department, Universidad Autónoma Agraria Antonio Narro, Saltillo, 25315, México
3 Health Area, Universidad La Salle Saltillo, Saltillo, 25298, México
4 Faculty of Engineering and Business San Quintin, Universidad Autónoma de Baja California, San Quintín, 22930, México
* Corresponding Author: Julio C. Chacón-Hernández. Email:
(This article belongs to this Special Issue: Plant Secondary Metabolites: Recent Advances and Opportunities)

Phyton-International Journal of Experimental Botany 2021, 90(3), 895-906. https://doi.org/10.32604/phyton.2021.014301

Received 16 September 2020; Accepted 04 January 2020; Issue published 30 March 2021

Abstract

The tomato (Solanum lycopersicum L.) is one of the world’s most important vegetable crops. Still, phytopathogenic bacteria affect the yield and quality of tomato cultivation, like Agrobacterium tumefeciens (At), Clavibacter michiganensis subsp. michiganensis (Cmm), Pseudomonas syringae pv. tomato (Pst), Ralstonia solanacearum (Rs), and Xanthomonas axonopodis (Xa). Synthetic chemical products are used mostly on disease plant control, but overuse generates resistance to bacterial control. This study aimed to evaluate the in vitro antibacterial activity of the ethanolic extract of Moringa oleifera Lam. leaves against At, Cmm, Pst, Rs, and Xa, as well as information about this plant species’ chemical composition. Antibacterial activity against pathogens observed by microplate technique, phytochemical screening, and FTIR analysis revealed different bio-active compounds on ethanolic extracts with antibacterial activity. The growth inhibition rate ranged between 0.08% and 99.94%. The inhibitory concentration, IC50, required to inhibit 50% of At, Cmm, Pst, Rs, and Xa bacterial growth, was 276.67, 350.48, 277.85, 351.49, and 283.22 mg/L, respectively. Inhibition of phytopathogen bacteria’s growth increased as the concentrations of the extract also increased. Moringa oleifera extract can be recommended as a potent bio-bactericide.

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Keywords

Biological control; secondary metabolites; tomato crop; inhibitory concentration; botanical extract

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Citations