Open Access

ARTICLE

Phenotype Analysis and Fine Mapping of the Male Sterile Mutant ms10 in Rice (Oryza sativa L.)

Gang Wei^#, Hongxia Yang^#, Jingwen Wu, Zixian Xiong, Nan Wang^*

College of Agronomy and Biotechnology, Rice Research Institute, Southwest University, Chongqing, 400715, China

* Corresponding Author: Nan Wang. Email:
# These authors contributed equally to this study

(This article belongs to the Special Issue: High-Yield Rice Physiology & Genetics)

Phyton-International Journal of Experimental Botany 2021, 90(3), 803-813. https://doi.org/10.32604/phyton.2021.014505

Received 03 October 2020; Accepted 16 November 2020; Issue published 30 March 2021

Abstract

There is a positive correlation between fertility and yield, and the decrease of fertility is bound to a greatly reduced crop yield. Male sterile mutants can be used in hybrid rice. Therefore, rice male sterility has an important value in research and application, and the study of related mutants is also very vital. The mutant ms10 (male sterile 10) reported in this study was induced by ethyl methane sulfonate (EMS) in the indica maintainer line Xinong 1B. There was no significant difference between the ms10 and wild type in the vegetative growth stage. However, in the reproductive growth stage, ms10 showed that the plant became shorter, the anther became smaller and the color became lighter, and finally showed the phenotype of male sterility in comparison to the wild type. I₂-KI staining showed that the pollen was malformed and only a little was active. Scanning electron microscopy observation showed that the exine waxy layer of the ms10 anther decreased, suggesting that the protective effect on pollen was decreased. This may be one of the reasons leading to the phenotype of male sterility. Finally, the pollen showed shrinkage and collapsed, and the structure of germinating pore cover disappeared. This may be the result of sterility. Genetic analysis showed that the male sterility phenotype of the mutant was controlled by a single recessive nuclear gene. MS10 was mapped between the molecular markers IND37 and IND51 on chromosome 4, with a physical distance of 178.6 kb. These results lay the foundation for further studies on MS10.

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