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Optimization of Factors Influencing Adventitious Rooting in Hybrid Larch

Kuipeng Li1,2, Hua Han1, Yunhui Xie1, Xiaomei Sun1,*
1 State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China
2 Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation & Key Laboratory of Central South Fast-Growing Timber Cultivation of Forestry Ministry of China, Guangxi Forestry Research Institute, Nanning, 530002, China
* Corresponding Author: Xiaomei Sun. Email:
(This article belongs to this Special Issue: Tree Somatic Embryogenesis and Application)

Phyton-International Journal of Experimental Botany 2021, 90(2), 583-593.

Received 26 August 2020; Accepted 21 September 2020; Issue published 07 February 2021


Optimization of in vitro adventitious root induction contributes to the development of a large-scale production system of hybrid clone seedlings of larch (Larix spp.). We used orthogonal testing to investigate the factors that affect the rooting of hybrid larch shoots–activated carbon, vitamin B1, sucrose, glycine, glutamic acid (Glu), cysteine, pH, and induction time in the dark and indole-3-butyric acid (IBA). Variance analysis showed that the effects of sucrose concentration on rooting rate, survival rate, and average root number, pH on the average number of roots; and Glu on survival rate were all significant (p < 0.05) when compared to the mock treatments. Among all of the 45 treatments, the highest rooting rate was 90%, the highest survival rate was 100%, and the highest average root number was 11.8. The theoretically optimal procedure of in vitro root induction of hybrid larch shoots is shoot induction on L9 modified medium (pH 6.7) containing 2 mg.L–1 naphthalene acetic acid, 0.25 mg.L–1 IBA, 1/3 macroelements, 7.5 g.L–1 sucrose, and 5 mg.L–1 Glu for 8 days, and then root induction on L9 modified medium without auxin.


Hybrid larch; adventitious root; orthogonal test; variance analysis

Cite This Article

Li, K., Han, H., Xie, Y., Sun, X. (2021). Optimization of Factors Influencing Adventitious Rooting in Hybrid Larch. Phyton-International Journal of Experimental Botany, 90(2), 583–593.

This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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