Open Access

ARTICLE

Efficient Evergreen Plant Regeneration of Cinnamomum japonicum Sieb. through in vitro Organogenesis

Jiaji Zhang¹, Xiaofei Long¹, Yuhao Weng¹, Tielong Cheng², Jisen Shi¹, Jinhui Chen^1,*
1 Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, 210037, China
2 College of Biology and the Environment, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, 210037, China
* Corresponding Author: Jinhui Chen. Email:
(This article belongs to this Special Issue: Tree Somatic Embryogenesis and Application)

Phyton-International Journal of Experimental Botany 2021, 90(2), 571-582. https://doi.org/10.32604/phyton.2021.014191

Received 08 September 2020; Accepted 09 September 2020; Issue published 07 February 2021

Abstract

Cinnamomum japonicum Sieb. is an excellent roadside tree and medicinal tree species with considerable ornamental and economic value. In this study, we successfully developed a large-scale micropropagation protocol for C. japonicum for the first time. Sterilized shoots were excised and used as explants for shoot induction on several basal media, supplemented with different concentrations of plant growth regulators (PGRs), such as Thidiazuron (TDZ), N⁶ -Benzyladenine (6-benzylaminopurine) (BA), α-naphthaleneacetic acid (NAA) and Gibberellic acid (GA₃). After comparison, the most efficient medium for shoot regeneration was 1/2 Murashige and Skoog (MS) medium containing 0.5 mg L^–1 BA, 0.05 mg L^–1 NAA and 0.2 mg L^–1 GA₃, which resulted in an average number of induced shoots per explant and shoot length of 5.2 and 1.62 cm at 28 d, respectively. Then, elongated adventitious shoots were transferred to induce roots. 86.7% of shoots was able to root on 1/2 MS medium supplemented with 0.5 mg L^–1 NAA and 0.1 mg L^–1 BA. The earliest rooting time observed was after 21 d and the average root length was up to 3.3 cm after 28 d. Our study shows that C. japonicum can be successfully regenerated through de novo organogenesis, which lays a foundation for future transformation research on this tree.

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Keywords

Cinnamomum japonicum Sieb.; micropropagation; de novo organogenesis; shoot induction; rhizogenesis

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