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ARTICLE
Establishment of Rhodiola quadrifida Hairy Roots and Callus Culture to Produce Bioactive Compounds
Anna Stepanova1,*, Maria Malunova1, Svetlana Salamaikina1, Renat Selimov2, Aleksandra Solov’eva1
1 Group of Specialized Root Metabolism, К.А. Timiryazev Institute of Plant Physiology RAS, Moscow, 127276, Russia
2 Bioanalytical laboratory, OOO “NVC Agrovetzaschita”, Moscow, 129329, Russia
* Corresponding Author: Anna Stepanova. Email:
(This article belongs to the Special Issue: Plant Secondary Metabolites: Recent Advances and Opportunities)
Phyton-International Journal of Experimental Botany 2021, 90(2), 543-552. https://doi.org/10.32604/phyton.2021.013223
Received 28 July 2020; Accepted 23 October 2020; Issue published 07 February 2021
Abstract
Rhodiola quadrifida is a rare mountain medicinal plant whose root
extracts are used in traditional Chinese medicine as a hemostatic, antitussive,
and tonic in the treatment of gynecological diseases. The aim of the study was
to obtain
R. quadrifida cultures at different degrees of differentiation
in vitro
and compare their growth characteristics and the content of salidroside and rosavin. Hairy roots were obtained by incubating cotyledons and hypocotyls in a suspension of
Agrobacterium rhizogenes strain A4. The presence of the rolB and
rolC genes was proven by polymerase chain reaction. The obtained roots were
cultivated in Murashige-Skoog medium (MS). Calluses were obtained from the
hairy roots in MS medium with the addition of hormones: 3 mg/L 2,4 D and
0.5 mg/L BAP. The presence of the main secondary metabolites of
R. quadrifida,
salidroside and rosavin, in calluses and salidroside in hairy roots by HPLC/MS
was confirmed. The content of salidroside in callus culture was significantly
higher than in hairy roots, 0.158 and 0.047%, respectively. The content of rosavin
in callus culture was 0.07%. The content of rosavin and salidroside in callus culture
was close to the level of these substances in the rhizomes of
R. quadrifida plants
growing
in vivo, making this culture promising for its possible biotechnological use.
Keywords
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APA Style
Stepanova, A., Malunova, M., Salamaikina, S., Selimov, R., Solov’eva, A. (2021). Establishment of rhodiola quadrifida hairy roots and callus culture to produce bioactive compounds. Phyton-International Journal of Experimental Botany, 90(2), 543-552. https://doi.org/10.32604/phyton.2021.013223
Vancouver Style
Stepanova A, Malunova M, Salamaikina S, Selimov R, Solov’eva A. Establishment of rhodiola quadrifida hairy roots and callus culture to produce bioactive compounds. Phyton-Int J Exp Bot. 2021;90(2):543-552 https://doi.org/10.32604/phyton.2021.013223
IEEE Style
A. Stepanova, M. Malunova, S. Salamaikina, R. Selimov, and A. Solov’eva "Establishment of Rhodiola quadrifida Hairy Roots and Callus Culture to Produce Bioactive Compounds," Phyton-Int. J. Exp. Bot., vol. 90, no. 2, pp. 543-552. 2021. https://doi.org/10.32604/phyton.2021.013223
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