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Establishment of Rhodiola quadrifida Hairy Roots and Callus Culture to Produce Bioactive Compounds

Anna Stepanova1,*, Maria Malunova1, Svetlana Salamaikina1, Renat Selimov2, Aleksandra Solov’eva1
1 Group of Specialized Root Metabolism, К.А. Timiryazev Institute of Plant Physiology RAS, Moscow, 127276, Russia
2 Bioanalytical laboratory, OOO “NVC Agrovetzaschita”, Moscow, 129329, Russia
* Corresponding Author: Anna Stepanova. Email:
(This article belongs to this Special Issue: Plant Secondary Metabolites: Recent Advances and Opportunities)

Phyton-International Journal of Experimental Botany 2021, 90(2), 543-552.

Received 28 July 2020; Accepted 23 October 2020; Issue published 07 February 2021


Rhodiola quadrifida is a rare mountain medicinal plant whose root extracts are used in traditional Chinese medicine as a hemostatic, antitussive, and tonic in the treatment of gynecological diseases. The aim of the study was to obtain R. quadrifida cultures at different degrees of differentiation in vitro and compare their growth characteristics and the content of salidroside and rosavin. Hairy roots were obtained by incubating cotyledons and hypocotyls in a suspension of Agrobacterium rhizogenes strain A4. The presence of the rolB and rolC genes was proven by polymerase chain reaction. The obtained roots were cultivated in Murashige-Skoog medium (MS). Calluses were obtained from the hairy roots in MS medium with the addition of hormones: 3 mg/L 2,4 D and 0.5 mg/L BAP. The presence of the main secondary metabolites of R. quadrifida, salidroside and rosavin, in calluses and salidroside in hairy roots by HPLC/MS was confirmed. The content of salidroside in callus culture was significantly higher than in hairy roots, 0.158 and 0.047%, respectively. The content of rosavin in callus culture was 0.07%. The content of rosavin and salidroside in callus culture was close to the level of these substances in the rhizomes of R. quadrifida plants growing in vivo, making this culture promising for its possible biotechnological use.


Rhodiola quadrifida; hairy roots; callus culture; HPLC; salidroside; rosavin

Cite This Article

Stepanova, A., Malunova, M., Salamaikina, S., Selimov, R., Solov’eva, A. (2021). Establishment of Rhodiola quadrifida Hairy Roots and Callus Culture to Produce Bioactive Compounds. Phyton-International Journal of Experimental Botany, 90(2), 543–552.


This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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