Open Access

ARTICLE

Inhibitory Effect of N, N-Dimethylhexadecylamine on the Growth of White-Rot Fungus Trametes versicolor (L.) in Wood

by Wilber Montejo-Mayo¹, Eduardo Días-Rivera¹, Mauro Martínez-Pacheco², Abril Munro-Rojas¹, Enrique Ambriz-Parra¹, Crisanto Velázquez-Becerra^1,*

1 Universidad Michoacana de San Nicolás de Hidalgo, Facultad de Ingeniería en Tecnología de la Madera, Ciudad Universitaria, Morelia, 58030, México
2 Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Ciudad Universitaria, Morelia, 58030, México

* Corresponding Author: Crisanto Velázquez-Becerra. Email:

Phyton-International Journal of Experimental Botany 2021, 90(1), 193-206. https://doi.org/10.32604/phyton.2020.011402

Received 07 May 2020; Accepted 14 July 2020; Issue published 20 November 2020

Abstract

Wood is an organic material that is a source of carbon of organisms called Wood-decay fungi, and to preserve the wood, various toxic compounds to man and the environment have been used. To analyze the effect of N,N-Dimethylhexadecylamine (DMHDA) on wood attacked by the rotting fungus Trametes versicolor L. We used an in vitro system to expose the fungus T. versicolor to different concentrations of the DMHDA (50, 150 and 450 μM). We quantified the diameter of mycelial growth and laccase activity, also, under these experimental conditions we studied morphological details of the organisms using different scanning equipment including scanning electron microscopy. The growth of T. versicolor exposed to DMHDA for 60 days, showed a concentration-dependent dose behavior, also, the electron microscopy analysis revealed that the morphology and mycelial density was affected by the DMHDA, showing a formation of atypical morphological and thickener folds. Finally, the pieces of wood treated with DMHDA and exposed to the fungus had a lower mass loss, after a period of 60 days of exposure, the values obtained were 0.7, 1.0 and 0.5 g of mass lost for the control, LoC and LoDMHDA treatments respectively. Wood-rot fungi have represented economic losses worldwide, the strategies used have been supported by toxic compounds for the environment. The DMHDA both in the Petri dish system and as a wood preservative was shown to significantly inhibit the growth of T. versicolor.

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