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ARTICLE
Characterization and Expression of Ammonium Transporter in Peach (Prunus persica) and Regulation Analysis in Response to External Ammonium Supply
Meiling Tang1,2,#, Yuhe Li1,3,#, Yahui Chen1,4, Lei Han1,3, Hongxia Zhang1,3, Zhizhong Song1,3,4,*
1 College of Agriculture, Ludong University, Yantai, 264025, China
2 Yantai Academy of Agricultural Sciences, Yantai, 264000, China
3 Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in Universities of Shandong (Ludong University), Yantai, 264025, China
4 Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, College of Forest, Nanjing Forest University, Nanjing, 214008, China
* Corresponding Author: Zhizhong Song. Email:
# These authors contributed equally to this work
Phyton-International Journal of Experimental Botany 2020, 89(4), 925-941. https://doi.org/10.32604/phyton.2020.011184
Received 24 April 2020; Accepted 29 May 2020; Issue published 09 November 2020
Abstract
As the preferred nitrogen (N) source, ammonium (NH
4+
) contributes to
plant growth and development and fruit quality. In plants, NH
4+ uptake is facilitated by a family of NH
4+ transporters (AMT). However, the molecular mechanisms and functional characteristics of the AMT genes in peach have not been
mentioned yet. In this present study, excess NH
4+ stress severely hindered shoot
growth and root elongation, accompanied with reduced mineral accumulation,
decreased leaf chlorophyll concentration, and stunned photosynthetic performance. In addition, we identified 14 putative AMT genes in peach (
PpeAMT).
Expression analysis showed that
PpeAMT genes were differently expressed in
peach leaves, stems and roots, and were distinctly regulated by external NH
4+
supplies. Putative
cis-elements involved in abiotic stress adaption, Ca
2+ response,
light and circadian rhythms regulation, and seed development were observed in
the promoters of the
PpeAMT family genes. Phosphorylation analysis of residues
within the C-terminal of PpeAMT proteins revealed many conserved phosphorylation
residues in both the AMT1 and AMT2 subfamily members, which could potentially
play roles in controlling the NH
4+ transport activities. This study provides gene
resources to study the biological function of AMT proteins in peach, and reveals
molecular basis for NH
4+ uptake and N nutrition mechanisms of fruit trees.
Keywords
Cite This Article
Tang, M., Li, Y., Chen, Y., Han, L., Zhang, H. et al. (2020). Characterization and Expression of Ammonium Transporter in Peach (
Prunus persica) and Regulation Analysis in Response to External Ammonium Supply.
Phyton-International Journal of Experimental Botany, 89(4), 925–941. https://doi.org/10.32604/phyton.2020.011184
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