Open Access

ARTICLE

High-Throughput MicroRNA and mRNA Sequencing Reveals that MicroRNAs May Be Involved in Pectinesterase-Mediated Cold Resistance in Potato

Chongchong Yan¹, Qianqian Wang¹, Nan Zhang², Jiajia Wang¹, Xuexiang Ren¹, Bingjie Xue³, Xiaojing Pu⁴, Zhihong Xu⁴, Huajun Liao^1,*

1 Anhui Academy of Agricultural Sciences, Hefei, 230031, China
2 Anhui Vocational College of City Management, Hefei, 231635, China
3 Funan County Agricultural Technology Promotion Center, Funan, 236300, China
4 Jieshou County Agricultural Technology Promotion Center, Jieshou, 236500, China

* Corresponding Author: Huajun Liao. Email:

(This article belongs to the Special Issue: Crop Production under Abiotic Stress: Physiological and Molecular Interventions)

Phyton-International Journal of Experimental Botany 2020, 89(3), 561-586. https://doi.org/10.32604/phyton.2020.010322

Received 27 February 2020; Accepted 28 April 2020; Issue published 22 June 2020

Abstract

Since potato cultivars are sensitive to low temperature, cold injury severely affects the geographical distribution and yield of potato. Although some miRNAs have been identified in response to cold stress in plants, there is no report about the role of miRNAs in the response to cold stress in potato. Here, via high throughput sequencing, we described the profiling of cold stress response to miRNA and mRNA in potato. Two small RNA and six mRNA libraries were constructed and sequenced. 296 known and 211 novel miRNAs were identified, in which 34 miRNAs in Cold Group (CG) had the higher expression quantity than which in Normal Group (NG) and 32 in CG had lower expression quantity than which in NG. 3068 differentially expressed genes were detected between NG and CG, in which 1400 genes were up-regulated and 1668 genes were down-regulated. The metabolism pathway of starch and sucrose (ko00500) is the common KEGG pathway in differentially expressed miRNA and mRNA. In this pathway, StuPME21575 and StuPME42971 are pectinesterase which mainly catalyzes the pectin-forming pectate, which are controlled by stu-miR6023 and stu-novel-miR42365. As the potato suffering cold stress, these two miRNAs expression levels became higher, but their target genes expression levels were just opposite and this result is the same with qRT-PCR.

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