Open Access

ARTICLE

Morphology, Phylogeny and Culture Characteristics of Ganoderma gibbosum Collected from Kunming, Yunnan Province, China

Thatsanee Luangharn^1,2,3,4, Samantha C. Karunarathna^1,3, Peter E. Mortimer^1,4,*, Kevin D. Hyde^3,5, Jianchu Xu^1,3,4,*

1 Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China
2 University of Chinese Academy of Sciences, Beijing, 100049, China
3 East and Central Asia Regional Office, World Agroforestry Centre (ICRAF), Kunming, 650201, China
4 Centre for Mountain Futures, Kunming Institute of Botany, Kunming, 650201, China
5 Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand

* Corresponding Authors: Peter E. Mortimer. Email: ; Jianchu Xu. Email:

Phyton-International Journal of Experimental Botany 2020, 89(3), 743-764. https://doi.org/10.32604/phyton.2020.09690

Received 15 January 2020; Accepted 04 March 2020; Issue published 22 June 2020

Abstract

Ganoderma is a genus of medicinally and economically important mushrooms in the family Ganodermataceae. Ganoderma species are popular medicinal mushrooms and their health benefits are well-documented. Ganoderma is a cosmopolitan genus that is widely distributed in both tropical and temperate regions. This genus is characterized by its unique laccate or non-laccate species with double-walled basidiospores. Here, we report on eight collections of G. gibbosum collected during surveys in Kunming, Yunnan Province, China. The specimens are described and illustrated based on macro- and micro-morphological characteristics. Total DNA of the eight G. gibbosum strains were extracted using the Biospin Fungal Extraction Kit following manufacturer protocol. Amplification of the Internal Transcribed Spacer (nrITS) region was carried out using ITS5/ITS4 primers and LROR/LR5 for the nuclear ribosomal large subunit 28S rDNA gene (LSU). Phylogenetic analysis with closely related species to G. gibbosum showed that all eight collections grouped with G. gibbosum with 100% bootstrap support. Phylogenetic similarity and morphological variations within the eight collections of G. gibbosum are discussed.

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