Open Access
ARTICLE
Genome-Wide Identification and Expression Profiling Suggest that Invertase Genes Function in Silique Development and the Response to Sclerotinia sclerotiorum in Brassica napus
Jingsen Liu1,2, Jinqi Ma1,2, Ai Lin1,2, Chao Zhang1,2, Bo Yang1,2, Liyuan Zhang1,2, Lin Huang1,2, Jiana Li1,2,*
1 Chongqing Rapeseed Engineering Research Center, College of Agronomy and Biotechnology, Chongqing, 400715, China
2 Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China
* Corresponding Author: Jiana Li. Email:
Phyton-International Journal of Experimental Botany 2020, 89(2), 253-273. https://doi.org/10.32604/phyton.2020.09334
Received 03 December 2019; Accepted 04 February 2020; Issue published 22 April 2020
Abstract
Invertase (INV), a key enzyme in sucrose metabolism, irreversibly catalyzes the hydrolysis of sucrose to glucose and fructose, thus playing important
roles in plant growth, development, and biotic and abiotic stress responses. In this
study, we identified 27 members of the
BnaINV family in
Brassica napus. We
constructed a phylogenetic tree of the family and predicted the gene structures,
conserved motifs,
cis-acting elements in promoters, physicochemical properties
of encoded proteins, and chromosomal distribution of the
BnaINVs. We also analyzed the expression of the
BnaINVs in different tissues and developmental stages
in the
B. napus cultivar Zhongshuang 11 using qRT-PCR. In addition, we analyzed RNA-sequencing data to explore the expression patterns of the
BnaINVs
in four cultivars with different harvest indices and in plants inoculated with the
pathogenic fungus
Sclerotinia sclerotiorum. We used WGCNA (weighted coexpression network analysis) to uncover
BnaINVregulatory networks. Finally,
we explored the expression patterns of several
BnaINV genes in cultivars with
long (Zhongshuang 4) and short (Ningyou 12) siliques. Our results suggest that
BnaINVs play important roles in the growth and development of rapeseed siliques
and the defense response against pathogens. Our findings could facilitate the
breeding of high-yielding
B. napus cultivars with strong disease resistance.
Keywords
Cite This Article
APA Style
Liu, J., Ma, J., Lin, A., Zhang, C., Yang, B. et al. (2020). Genome-wide identification and expression profiling suggest that invertase genes function in silique development and the response to sclerotinia sclerotiorum in brassica napus. Phyton-International Journal of Experimental Botany, 89(2), 253-273. https://doi.org/10.32604/phyton.2020.09334
Vancouver Style
Liu J, Ma J, Lin A, Zhang C, Yang B, Zhang L, et al. Genome-wide identification and expression profiling suggest that invertase genes function in silique development and the response to sclerotinia sclerotiorum in brassica napus. Phyton-Int J Exp Bot. 2020;89(2):253-273 https://doi.org/10.32604/phyton.2020.09334
IEEE Style
J. Liu et al., "Genome-Wide Identification and Expression Profiling Suggest that Invertase Genes Function in Silique Development and the Response to Sclerotinia sclerotiorum in Brassica napus," Phyton-Int. J. Exp. Bot., vol. 89, no. 2, pp. 253-273. 2020. https://doi.org/10.32604/phyton.2020.09334